三维打印含铜钛合金对骨髓间充质干细胞成骨分化的影响

doi:10.19438/j.cjoms.2022.04.004

中国口腔颌面外科杂志 ›› 2022, Vol. 20 ›› Issue (4): 334-339.doi: 10.19438/j.cjoms.2022.04.004

三维打印含铜钛合金对骨髓间充质干细胞成骨分化的影响

蒋胜杰^*, 梅鹏^*, 周皓楠, 潘兆澄, 高源, 蔡鸣

上海交通大学医学院附属第九人民医院口腔颅颌面科,上海交通大学口腔医学院,国家口腔医学中心, 国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海 200011

收稿日期:2022-03-03 修回日期:2022-04-17 出版日期:2022-07-20 发布日期:2022-07-20
通讯作者: 蔡鸣,E-mail: zidanecm500@126.com
作者简介:蒋胜杰（1995-）,男,硕士研究生,E-mail: jsj960419@163.com;梅鹏（1996-）,男,硕士研究生,E-mail: meip1217@163.com。^*并列第一作者
基金资助:
上海市自然科学基金（20ZR1431000）

Cu-bearing titanium alloy based on three-dimensional printing promoted osteogenic differentiation of bone marrow mesenchymal stem cells

JIANG Sheng-jie, MEI Peng, ZHOU Hao-nan, PAN Zhao-cheng, GAO Yuan, CAI Ming

Department of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2022-03-03 Revised:2022-04-17 Online:2022-07-20 Published:2022-07-20

摘要/Abstract

摘要： 目的: 探讨三维打印含铜钛合金的体外生物相容性和对骨髓间充质干细胞（bone marrow mesenchymal stem cells,BMSCs）成骨分化的影响。方法: 以Ti-6Al-4V-5Cu合金粉末为原料,通过选择性激光熔化（selective laser melting, SLM）技术制备三维打印含铜钛合金（3D Ti-5Cu）;将BMSCs与3D Ti-5Cu合金共同培养,观察其对细胞活性、黏附、增殖、碱性磷酸酶（ALP）活性及成骨分化相关基因表达的影响,以医用钛合金（Ti-6Al-4V）作为对照组。采用 GraphPad Prism 9.3.1软件包对数据进行统计学分析。结果: 成功制备3D Ti-5Cu合金,该新型合金无细胞毒性,与对照组相比无统计学差异（P>0.05）。3D Ti-5Cu合金能促进BMSCs的早期黏附、增殖,并显著提高ALP活性和Runx2、Alp、Bmp2、Opn及Col1a1等成骨分化基因的表达水平（P<0.05）。结论: 3D Ti-5Cu合金具备出色的生物相容性,能促进BMSCs的成骨分化,有利于改善钛合金的生物惰性,为实现钛合金的生物功能化提供了理论依据。

关键词: 三维打印, 含铜钛合金, 骨髓间充质干细胞, 成骨分化

Abstract: PURPOSE: To explore the biocompatibility and the properties to enhance osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) by Cu-bearing titanium alloy based on three-dimensional printing. METHODS: Cu-bearing titanium alloy based on three-dimensional printing(3D Ti-5Cu) was fabricated using Ti-6Al-4V-5Cu alloy powders as raw material via selective laser melting(SLM) technology. The 3D Ti-5Cu alloy was co-cultured with BMSCs to observe its effect on cell activity, adhesion, proliferation, alkaline phosphatase(ALP) activity and gene expression related to osteogenic differentiation, medical titanium alloy (Ti-6Al-4V) was set as the control group. The data were analyzed with GraphPad Prism 9.3.1 software. RESULTS: 3D Ti-5Cu alloy with good cytocompatibility was successfully fabricated in this study, and no statistical difference was found compared with the control group(P>0.05). Meanwhile, 3D Ti-5Cu alloy could enhance adhesion and proliferation of BMSCs, and significantly increased ALP activity and the expression levels of osteogenic differentiation genes such as Runx2, Alp, Bmp2, Opn and Col1a1, and significant differences were found between the groups(P<0.05). CONCLUSIONS: 3D Ti-5Cu alloy possesses excellent biocompatibility and can further promote the osteogenic differentiation of BMSCs, which is beneficial to improve the biological inertia of titanium alloys, and provides a theoretical basis for the realization of biological functionalization of titanium alloys.

Key words: Three-dimensional printing, Cu-bearing titanium alloy, Bone marrow mesenchymal stem cells, Osteogenic differentiation

中图分类号:

R782

蒋胜杰, 梅鹏, 周皓楠, 潘兆澄, 高源, 蔡鸣. 三维打印含铜钛合金对骨髓间充质干细胞成骨分化的影响[J]. 中国口腔颌面外科杂志, 2022, 20(4): 334-339.

JIANG Sheng-jie, MEI Peng, ZHOU Hao-nan, PAN Zhao-cheng, GAO Yuan, CAI Ming. Cu-bearing titanium alloy based on three-dimensional printing promoted osteogenic differentiation of bone marrow mesenchymal stem cells[J]. China J Oral Maxillofac Surg, 2022, 20(4): 334-339.

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三维打印含铜钛合金对骨髓间充质干细胞成骨分化的影响

Cu-bearing titanium alloy based on three-dimensional printing promoted osteogenic differentiation of bone marrow mesenchymal stem cells

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