唾液腺恶性多形性腺瘤中CDH1基因启动子甲基化与E-cadherin表达的相关性分析

doi:10.19438/j.cjoms.2018.02.005

中国口腔颌面外科杂志 ›› 2018, Vol. 16 ›› Issue (2): 120-125.doi: 10.19438/j.cjoms.2018.02.005

唾液腺恶性多形性腺瘤中CDH1基因启动子甲基化与E-cadherin表达的相关性分析

夏亮^1,2, 胡宇华¹, 王丽珍¹, 顾挺¹, 李江¹, 田臻¹

1.上海交通大学医学院附属第九人民医院·口腔医学院口腔病理科, 2.口腔颅颌面外科, 上海市口腔医学重点实验室,上海市口腔医学研究所,国家口腔疾病临床研究中心,上海 200011

收稿日期:2017-10-22 修回日期:2017-12-25 出版日期:2018-03-20 发布日期:2018-04-08
通讯作者: 田臻,E-mail：tian0304_cn@163.com
作者简介:夏亮（1990-）,男,E-mail：anglexialiang@126.com
基金资助:
国家自然科学基金（81272976、81372910）

CDH1 gene methylation and E-cadherin in salivary malignant pleomorphic adenoma

XIA Liang^1,2, HU Yu-hua¹, WANG li-zhen¹, GU Ting¹, LI Jiang¹, TIAN Zhen¹

1.Department of Oral Pathology, 2.Department of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology; National Clinical Research Center of Stomatology. Shanghai 200011, China;

Received:2017-10-22 Revised:2017-12-25 Online:2018-03-20 Published:2018-04-08

摘要/Abstract

摘要： 目的: 分析唾液腺恶性多形性腺瘤（malignant pleomorphic adenoma,MPA）中CDH1启动子甲基化程度与E-cadherin表达的相关性,评估CDH1启动子甲基化在E-cadherin沉默中的作用,并探讨其与MPA临床病理指标之间的相关性,对患者预后的潜在评估价值。 方法: 采用免疫组织化学法、BSP法分别检测37例MPA临床标本中E-cadherin的表达及CDH1启动子甲基化程度,进行相关性分析。收集患者资料,进行临床随访,分析CDH1甲基化与患者临床病理指标及生存率之间的相关性。采用SPSS 16.0软件包进行统计学分析。 结果: CDH1高甲基化与E-cadherin沉默表达呈显著正相关。CDH1甲基化程度与患者性别、肿瘤分化类型、组织学分级、淋巴结转移及TNM分期显著相关。CDH1高甲基化者生存率差,淋巴结转移为MPA患者预后的独立预测因子。 结论: CDH1基因高甲基化是MPA中E-cadherin沉默表达的重要调控机制之一,CDH1甲基化有望作为评估MPA患者临床预后的预测因子之一。

关键词: E-cadherin, DNA甲基化, 恶性多形性腺瘤

Abstract: PURPOSE: To analyze the correlation between CDH1 promoter methylation and E-cadherin expression in MPA, evaluate the role of CDH1 promoter methylation in E-cadherin silencing, explore the correlation between CDH1 methylation and clinicopathological parameters of MPA, and assess its potential prognostic value. METHODS: Immunohistochemistry and BSP were used to detect the expression of E-cadherin and the degree of CDH1 promoter methylation in MPA samples, and correlation analysis was performed. The data of patients were collected and followed up, and the correlation between CDH1 methylation and clinical pathological parameters and survival rate was analyzed. SPSS16.0 software package was used for statistical analysis. RESULTS: CDH1 hypermethylation was positively correlated with E-cadherin silencing expression. The degree of CDH1 methylation was significantly correlated with gender, tumor differentiation, histological grade, lymph node metastasis and TNM staging. The survival rate of patients with CDH1 hypermethylation was poor. Lymph node metastasis was an independent predictor of prognosis in patients with MPA. CONCLUSIONS: CDH1 gene hypermethylation is one of the important regulatory mechanisms of E-cadherin silencing in MPA. CDH1 methylation may be one of the predictors for clinical prognosis in patients with MPA.

Key words: E-cadherin, DNA methylation, Malignant pleomorphic adenoma

中图分类号:

R739.8

夏亮, 胡宇华, 王丽珍, 顾挺, 李江, 田臻. 唾液腺恶性多形性腺瘤中CDH1基因启动子甲基化与E-cadherin表达的相关性分析[J]. 中国口腔颌面外科杂志, 2018, 16(2): 120-125.

XIA Liang, HU Yu-hua, WANG li-zhen, GU Ting, LI Jiang, TIAN Zhen. CDH1 gene methylation and E-cadherin in salivary malignant pleomorphic adenoma[J]. China J Oral Maxillofac Surg, 2018, 16(2): 120-125.

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唾液腺恶性多形性腺瘤中CDH1基因启动子甲基化与E-cadherin表达的相关性分析

CDH1 gene methylation and E-cadherin in salivary malignant pleomorphic adenoma

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