MCM7及HPV在上颌窦鳞状细胞癌中的表达及临床意义

doi:10.19438/j.cjoms.2017.05.003

中国口腔颌面外科杂志 ›› 2017, Vol. 15 ›› Issue (5): 392-396.doi: 10.19438/j.cjoms.2017.05.003

MCM7及HPV在上颌窦鳞状细胞癌中的表达及临床意义

胡月¹，卢利²，姜菲菲¹，丁晓旭¹，王佳说¹，阎艾慧¹

1.中国医科大学附属第一医院耳鼻咽喉科，辽宁沈阳 110001;
2.中国医科大学附属口腔医院口腔颌面外科，辽宁沈阳 110001

收稿日期:2016-10-26 出版日期:2017-08-30 发布日期:2017-10-27
通讯作者: 阎艾慧，E-mail:yah567@sina.com
作者简介:胡月（1990-），男，住院医师，E-mail：hy199065@sina.com
基金资助:
辽宁省科技厅科学技术计划项目（2014225020）

Expression and clinical significance of MCM7 and HPV in squamous cell carcinoma of the maxillary sinus

HU Yue¹, LU Li², JIANG Fei-fei¹, DING Xiao-xu¹, WANG Jia-shuo¹, YAN Ai-hui¹.

1.Department of Otorhinolaryngology, the First Hospital of China Medical University. Shenyang 110001;
2.Department of Oral and Maxillofacial Surgery, China Medical University Stomatological Hospital. Shenyang 110001, Liaoning Province, China

Received:2016-10-26 Online:2017-08-30 Published:2017-10-27

摘要/Abstract

摘要： 目的：通过检测MCM7和HPV在上颌窦鳞癌（squamous cell carcinoma of the maxillary sinus，MSSCC）中的表达，探讨其在MSSCC发病机制中的作用及临床意义。方法：采用免疫组织化学方法检测MSSCC（实验组）、癌旁组织（癌旁组）和下鼻甲黏膜组织（对照组）中的MCM7及HPV的表达，应用SPSS 22.0 软件包对数据进行统计学分析。结果：①MCM7在癌旁组和对照组上皮中大部分位于基底层，而其在MSSCC中呈弥散分布。MCM7在对照组（P=0.000）、癌旁组（P=0.000）和实验组（P=0.000）中的表达水平逐渐增高。②MCM7的表达仅与肿瘤分化程度有关（P=0.030），并且随着分化程度的下降，MCM7的表达水平逐渐增高。③伴HPV感染的细胞无论在对照组、癌旁组或实验组中分布均无明显规律。HPV在对照组、癌旁组及实验组患者中的感染率分别为40.00%（4/10）、95.65%（22/23）和78.57%（33/42）。④HPV感染与颈淋巴结转移有关（P=0.015），而与患者性别（P=1.000）、年龄（P=0.332）、吸烟（P=0.536）、临床分期（P=0.806）及肿瘤分化程度（P=0.489）无关。⑤在实验组中，伴HPV感染者，MCM7表达水平显著高于未感染者（P=0.007），且呈正相关关系（rs=0.317, P=0.041）。结论：MCM7和HPV可能与MSSCC的发生、发展有关，可能对患者的临床治疗及预后判断具有重要指导意义； MCM7异常表达可作为筛查HPV感染病例的良好临床病理学指标。

关键词: 上颌窦, 鳞状细胞癌, 微小染色体维持蛋白7, 人乳头瘤病毒

Abstract: PURPOSE: This study was designed to investigate the expression of minichromosome maintenance protein 7(MCM7) and human papillomavirus (HPV) in squamous cell carcinoma of the maxillary sinus (MSSCC) and its relationship with the pathogenesis of this disease and clinical significance. METHODS: Using immunohistochemistry method, we studied the expression of MCM7 and HPV in MSSCC (experimental group), corresponding adjacent tissues (adjacent group) and inferior turbinate mucosal tissues (control group). SPSS 22.0 software package was used to analyze the data. RESULTS: MCM7 was located in the basal layer of the epithelium in the adjacent group and the control group, but diffusedly distributed in the MSSCC. The expression of MCM7 in control (P=0.000), adjacent (P=0.000) and experimental (P=0.000) group increased gradually. The expression of MCM7 was only related to the degree of tumor differentiation (P=0.030), and the expression of MCM7 increased gradually with the decrease of the degree of differentiation. The cells infected with HPV had no obvious regularity in the control, the adjacent or the experimental group. The infection rates of HPV in the control group， the adjacent group and the experimental group were 40.00%（4/10）,95.65%（22/23）,78.57%（33/42）, respectively. HPV infection was associated with cervical lymph node metastasis (P=0.015), but not gender (P=1.000), age (P=0.332), smoking (P=0.536), clinical stage (P=0.806) and tumor differentiation (P=0.489). The expression level of MCM7 in patients with HPV infection was significantly higher than that in the uninfected patients (P=0.007), and there was a positive correlation between them (rs=0.317, P=0.041). CONCLUSIONS: MCM7 and HPV maybe correlate with the occurrence and development of MSSCC, which may have important significance for clinical treatment and prediction of prognosis.

Key words: Maxillary sinus, Squamous cell carcinoma, Minichromosome maintenance protein 7, Human papillomavirus

中图分类号:

R739.8 [文献标志码] A

胡月，卢利，姜菲菲，丁晓旭，王佳说，阎艾慧. MCM7及HPV在上颌窦鳞状细胞癌中的表达及临床意义[J]. 中国口腔颌面外科杂志, 2017, 15(5): 392-396.

HU Yue, LU Li, JIANG Fei-fei, DING Xiao-xu, WANG Jia-shuo, YAN Ai-hui.. Expression and clinical significance of MCM7 and HPV in squamous cell carcinoma of the maxillary sinus[J]. China J Oral Maxillofac Surg, 2017, 15(5): 392-396.

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MCM7及HPV在上颌窦鳞状细胞癌中的表达及临床意义

Expression and clinical significance of MCM7 and HPV in squamous cell carcinoma of the maxillary sinus

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