头颈鳞癌患者源性类器官模型的建立和鉴定

doi:10.19438/j.cjoms.2022.04.003

中国口腔颌面外科杂志 ›› 2022, Vol. 20 ›› Issue (4): 328-333.doi: 10.19438/j.cjoms.2022.04.003

头颈鳞癌患者源性类器官模型的建立和鉴定

汪冲, 康馨丹, 王瑞, 吴亚平, 孙露露, 张志愿, 孙树洋

上海交通大学医学院附属第九人民医院口腔颌面-头颈肿瘤科,上海交通大学口腔医学院, 国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海 200011

收稿日期:2021-12-21 修回日期:2022-04-17 出版日期:2022-07-20 发布日期:2022-07-20
通讯作者: 孙树洋,E-mail: sunshuyang@sjtu.edu.cn
作者简介:汪冲（1996-）,男,硕士研究生,E-mail: wangchongkq@sjtu.edu.cn
基金资助:
国家自然科学基金面上项目（81872199）; 国家重点研发计划（2017YFC0908500）; 国家自然科学基金重点项目（82030085）

Establishment and identification of patient-derived organoid model in head and neck squamous carcinoma

WANG Chong, KANG Xin-dan, WANG Rui, WU Ya-ping, SUN Lu-lu, ZHANG Zhi-yuan, SUN Shu-yang

Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011,China

Received:2021-12-21 Revised:2022-04-17 Online:2022-07-20 Published:2022-07-20

摘要/Abstract

摘要： 目的: 构建及鉴定头颈鳞癌（head and neck squamous cell carcinoma, HNSCC）患者源性类器官（patient-derived organoid, PDO）模型。方法: 通过HNSCC样本分离肿瘤细胞,于基质胶中三维培养和传代,定时记录模型生长情况。利用H-E染色观察PDO模型与患者肿瘤组织的组织病理学形态,同时利用免疫组织化学染色检测HNSCC标志物CKH、P63、SMA和Ki67在两者间的表达情况,评估PDO模型与患者肿瘤组织间的符合程度。结果: 本研究建立3例能稳定传代的PDO模型,组织病理学及4个肿瘤标志物鉴定结果显示模型与患者组织间一致性较高。结论: 以HNSCC样本构建的PDO模型能够反映患者的组织形态学和生物标志物特征,可作为今后HNSCC研究中可靠的临床前模型。

关键词: 头颈鳞癌, 患者源性类器官模型, 临床前模型

Abstract: PURPOSE: To construct and identify patient-derived organoid (PDO) model of head and neck squamous cell carcinoma(HNSCC). METHODS: Tumor cells, isolated from HNSCC samples, were cultured and passaged in matrix gel with three dimensions. The growth was also recorded at regular intervals. The correlation between patient tumor tissue and PDO models in histopathological and tumor molecular markers was compared using H-E and immunohistochemical staining assay. RESULTS: This study displayed 3 cases of PDO models that could be passaged stably, and the histopathology and tumor marker identification results showed that the model and the patient's tissues were consistent with each other. CONCLUSIONS: The PDO model constructed from samples of HNSCC could reflect the patient's histomorphology and biomarker characteristics, and could be utilized as a reliable preclinical model in the further HNSCC study.

Key words: Head and neck squamous cell carcinoma, Patient-derived organoid, Preclinical model

中图分类号:

R739.8

汪冲, 康馨丹, 王瑞, 吴亚平, 孙露露, 张志愿, 孙树洋. 头颈鳞癌患者源性类器官模型的建立和鉴定[J]. 中国口腔颌面外科杂志, 2022, 20(4): 328-333.

WANG Chong, KANG Xin-dan, WANG Rui, WU Ya-ping, SUN Lu-lu, ZHANG Zhi-yuan, SUN Shu-yang. Establishment and identification of patient-derived organoid model in head and neck squamous carcinoma[J]. China J Oral Maxillofac Surg, 2022, 20(4): 328-333.

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头颈鳞癌患者源性类器官模型的建立和鉴定

Establishment and identification of patient-derived organoid model in head and neck squamous carcinoma

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