热疗联合Id-1基因沉默对舌鳞癌CAL-27细胞增殖及侵袭的影响

doi:10.19438/j.cjoms.2018.06.002

中国口腔颌面外科杂志 ›› 2018, Vol. 16 ›› Issue (6): 488-492.doi: 10.19438/j.cjoms.2018.06.002

热疗联合Id-1基因沉默对舌鳞癌CAL-27细胞增殖及侵袭的影响

罗丹¹, 孙巧珍¹, 刘少华², 孙俊伟³, 王升志¹

1.青岛大学附属烟台毓璜顶医院口腔颌面外科,山东烟台 264000;
2.山东大学齐鲁医院口腔颌面外科,山东济南 250012;
3.烟台业达医院口腔颌面外科,山东烟台 264000

收稿日期:2018-06-14 出版日期:2018-11-20 发布日期:2019-01-11
通讯作者: 王升志,E-mail：wangsz916@163.com
作者简介:罗丹（1992-）,女,在读硕士研究生,E-mail：kqluodan@163.com
基金资助:
山东省科技发展计划项目（2014GSF118184）

Effect of hyperthermia combined with silencing Id-1 gene on proliferation and invasion of tongue squamous cell carcinoma cell line CAL-27

LUO Dan¹, SUN Qiao-zhen¹, LIU Shao-hua², SUN Jun-wei³, WANG Sheng-zhi¹

1.Department of Oral and Maxillofacial Surgery, Affiliated Yantai Yuhuangding Hospital of Qingdao University. Yantai 264000;
2.Department of Oral and Maxillofacial Surgery, Qilu Hospital of Shandong University. Jinan 250012;
3. Department of Oral and Maxillofacial Surgery, Yantai Yeda Hospital. Yantai 264000, Shandong Province, China

Received:2018-06-14 Online:2018-11-20 Published:2019-01-11

摘要/Abstract

摘要： 目的: 探讨热疗联合Id-1基因沉默对舌鳞癌细胞增殖及侵袭的影响及其机制。方法: 将培养的舌鳞癌细胞株CAL-27分为4组。①沉默Id-1组（Id-1-siRNA）—用siRNA基因转染法沉默舌鳞癌CAL-27细胞中Id-1的表达,RT-PCR法检测其Id-1的mRNA表达变化;②热疗组（HT）—细胞置于42.5℃恒温培养箱内40min;③联合组（HT+ Id-1-siRNA）—siRNA基因转染法沉默细胞内Id-1的表达后进行42.5℃、40 min的热疗处理;④空白对照组—常规培养细胞。通过CCK8和Transwell侵袭实验,分别检测CAL-27细胞的增殖能力和侵袭能力变化; Western免疫印迹法检测CAL-27细胞中PI3K、磷酸化Akt（p-Akt）蛋白表达变化。采用SPSS19.0软件包对数据进行统计学分析。结果: siRNA能成功沉默CAL-27细胞内Id-1的表达,沉默Id-1组与空白对照组相比,Id-1的mRNA表达水平下降81.6%。沉默Id-1和热疗均可抑制CAL-27细胞的增殖和侵袭能力,下调PI3K、p-Akt蛋白表达（P<0.05）,热疗联合沉默Id-1作用于CAL-27细胞后的结果更为显著（P<0.01）。结论: 热疗协同沉默Id-1可显著抑制人舌鳞癌细胞的增殖及侵袭能力,其作用是通过PI3K/Akt信号通路来实现。

关键词: 舌鳞状细胞癌, 热疗, Id-1, 增殖, 侵袭, PI3K/Akt信号通路

Abstract: PURPOSE: To investigate the effects and regulatory mechanisms of hyperthermia combined with silencing Id-1 gene on proliferation and invasion of tongue squamous cell carcinoma in vitro. METHODS: Cal-27 cells were divided into 4 groups. In silencing Id-1 group (Id-1-siRNA), siRNA was used to silence the expression of Id-1 in the CAL-27 cells, and the mRNA expression of Id-1 was detected by RT-PCR. In hyperthermia group (HT), CAL-27 cells were placed into constant temperature incubator at 42.5℃ for 40 minutes. In combined group (HT+Id-1-siRNA), hyperthermia combined with silencing Id-1 gene were exerted on CAL-27 cells. Normally cultured CAL-27 cells were used as control group. The proliferation and invasion ability were analyzed by CCK8 assay and Transwell chamber test,and the protein expression of PI3K, p-Akt was detected by Western blotting. SPSS 19.0 software package was used to analyze the data. RESULTS: The expression level of Id-1 mRNA decreased by 81.6% in the Id-1-siRNA group, compared with the blank control group. Both silencing Id-1 and hyperthermia not only inhibited the proliferation and invasion of CAL-27 cells（P<0.05）, but also down-regulated the protein expression of PI3K, p-Akt; however, the combination of hyperthermia and Id-1-siRNA contributed to more significant results. CONCLUSIONS: Hyperthermia in conjunction with silencing Id-1 could reduce the proliferation and invasion of CAL-27 cell line of tongue squamous cell carcinoma by down-regulating PI3K/Akt signaling pathway.

Key words: Tongue squamous cell carcinoma, Hyperthermia, Inhibitor of differentiation/DNA binding-1, Proliferation, Invasion, PI3K/Akt signaling pathway

中图分类号:

R739.8

罗丹, 孙巧珍, 刘少华, 孙俊伟, 王升志. 热疗联合Id-1基因沉默对舌鳞癌CAL-27细胞增殖及侵袭的影响[J]. 中国口腔颌面外科杂志, 2018, 16(6): 488-492.

LUO Dan, SUN Qiao-zhen, LIU Shao-hua, SUN Jun-wei, WANG Sheng-zhi. Effect of hyperthermia combined with silencing Id-1 gene on proliferation and invasion of tongue squamous cell carcinoma cell line CAL-27[J]. China J Oral Maxillofac Surg, 2018, 16(6): 488-492.

参考文献

[1] Sano D, Myers JN.Metastasis of squamous cell carcinoma of the oral tongue[J]. Cancer Metastasis Rev, 2007, 26(3-4): 645-662.
[2] Kim W, Kim M S, Kim H, et al.Role of HIF-1α in response of tumors to a combination of hyperthermia and radiation in vivo[J]. Int J Hyperthermia, 2018, 34(3): 276-283.
[3] Mitsudo K, Koizumi T, Iida M, et al.Thermochemoradiation therapy using superselective intra-arterial infusion via superficial temporal and occipital arteries for oral cancer with N3 cervical lymph node metastases[J]. Int J Radiat Oncol Biol Phys, 2012, 83(5): e639-645.
[4] Tang YL, Jiang J, Liu J, et al.Hyperthermia inhibited the migration of tongue squamous cell carcinoma through TWIST2[J]. J Oral Pathol Med, 2015, 44(5): 337-344.
[5] Ling F, Kang B, Sun XH.Id proteins: small molecules, mighty regulators[J]. Curr Top Dev Biol, 2014, 110:189-216.
[6] 郭斌, 张成梅, 刘少华. Id-1在恶性肿瘤发生中的作用及其机制[J]. 生命科学, 2010, 22(5): 401-404.
[7] Roschger C, Cabrele C.The Id-protein family in developmental and cancer-associated pathways[J]. Cell Commun Signal, 2017, 15(1): 7-32.
[8] Issels RD.Hyperthermia adds to chemotherapy[J]. Eur J Cancer, 2008, 44(17): 2546-2554.
[9] Shetake NG, Kumar A, Gaikwad S, et al.Magnetic nanoparticle-mediated hyperthermia therapy induces tumour growth inhibition by apoptosis and Hsp90/AKT modulation[J]. Int J Hyperther, 2015, 31(8): 909-919.
[10] Hatashita M, Taniguchi M, Baba K, et al.Sinodielide A exerts thermosensitizing effects and induces apoptosis and G2/M cell cycle arrest in DU145 human prostate cancer cells via the Ras/Raf/MAPK and PI3K/Akt signaling pathways[J]. Int J Mol Med, 2014, 33(2): 406-414.
[11] Zhao J, Wang S, Liu N, et al.Correlation between the expression of Id-1 and hyperthermia-associated molecules in oral squamous cell carcinoma[J]. J Clin Pathol, 2013, 66(9): 758-763.
[12] Ling MT, Wang X, Zhang X, et al.The multiple roles of Id-1 in cancer progression[J]. Differentiation, 2006, 74(9-10): 481-487.
[13] 王丹, 陈晓, 徐葳, 等. 自分泌IL-6经Ras/MEK/ERK、PI3K/Akt通路促进卵巢癌细胞黏附和侵袭功能的研究[J]. 免疫学杂志, 2016, 32(4): 294-298.
[14] Danielsen SA, Eide PW, Nesbakken A, et al.Portrait of the PI3K/AKT pathway in colorectal cancer[J]. Biochim Biophys Acta, 2015, 1855(1): 104-121.
[15] Li B, Cheung P Y, Wang X, et al.Id-1 activation of PI3K/Akt/NFκB signaling pathway and its significance in promoting survival of esophageal cancer cells[J]. Carcinogenesis, 2007, 28(11): 2313-2320.
[16] Hao L, Liao Q, Tang Q, et al.Id-1 promotes osteosarcoma cell growth and inhibits cell apoptosis via PI3K/AKT signaling pathway[J]. Biochem Biophys Res Commun, 2016, 470(3): 643-649.
[17] 曾益新, 张晓实, 刘强. 分子靶向治疗:肿瘤治疗的里程碑[J]. 癌症, 2008, 27(8):785-787.

热疗联合Id-1基因沉默对舌鳞癌CAL-27细胞增殖及侵袭的影响

Effect of hyperthermia combined with silencing Id-1 gene on proliferation and invasion of tongue squamous cell carcinoma cell line CAL-27

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