Twist1调控上皮-间质转化促进舌鳞癌细胞顺铂耐药机制的研究

doi:10.19438/j.cjoms.2018.01.001

中国口腔颌面外科杂志 ›› 2018, Vol. 16 ›› Issue (1): 1-5.doi: 10.19438/j.cjoms.2018.01.001

Twist1调控上皮-间质转化促进舌鳞癌细胞顺铂耐药机制的研究

刘墨¹, 阮毅¹, 李劲松¹, 黄洪章²

1.中山大学孙逸仙纪念医院口腔科,广东广州 510120;
2.中山大学光华口腔医学院口腔颌面外科,广东广州 510055

收稿日期:2017-06-12 修回日期:2017-08-29 出版日期:2018-01-20 发布日期:2018-02-11
通讯作者: 黄洪章,E-mail:huanghongzhang@tom.com
作者简介:刘墨(1978-),女,博士,副主任医师,E-mail：mmliumo@163.com
基金资助:
国家自然科学基金 (81502350）; 广东省自然基础与应用基础研究专项（广东省自然科学基金）自由申请项目（2016A030313352）

Study on Twist1 promoting epithelial-mesenchymal transition and drug-resistance in tongue squamous cell carcinoma

LIU Mo¹, RUAN Yi¹, LI Jing-song¹, HUANG Hong-zhang²

1.Department of Stomatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University. Guangzhou 510120;
2 Department of Oral and Maxillofacial Surgery, Guanghua School and Research Institute of Stomatology, Sun Yat-sen University.Guangzhou 510055, Guangdong Province, China

Received:2017-06-12 Revised:2017-08-29 Online:2018-01-20 Published:2018-02-11

摘要/Abstract

摘要： 目的: 探讨Twist1调控上皮-间质转化（EMT）促进舌鳞状细胞癌细胞顺铂耐药的机制。方法: 在CAL27/CDDP中转染Twist1 siRNA,免疫印迹及免疫荧光检测E-cadherin、Vimentin的表达;免疫印迹检测Twist1、Slug的表达;Transwell及划痕实验检测细胞的侵袭、迁移能力;MTT检测细胞对顺铂的半抑制率。采用SPSS17.0 软件包对结果进行单因素方差分析。结果: 转染Twist1 siRNA可逆转CAL27/CDDP的EMT表型, E-cadherin表达增强,Vimentin、Twist1及Slug表达降低,细胞的侵袭、迁移能力明显降低,IC₅₀下降41.75%±5.10%（P<0.01）。结论: Twist1可通过调控上皮-间质转化而促进舌鳞癌细胞的顺铂耐药。

关键词: 舌鳞状细胞癌, 上皮-间质转化, Twist1, 耐药性

Abstract: PURPOSE: The aim of this study was to investigate the mechanism of Twist1 regulating epithelial-mesenchymal transition (EMT) and cisplatin-resistance in tongue squamous carcinoma cells. METHODS: TSCC CDDP resistant cell line CAL27/CDDP was transfected with Twist1 siRNA. Western blot and immunofluorescence staining were used to detect the expression of E-cadherin and Vimentin; Western blot was used to detect the expression of Twist1 and Slug; Transwell assay and scratch test were used to detect the invasion and migration capabilities; MTT was used to analyze the half maximal inhibitory concentration (IC₅₀) values. One-way ANOVA was used to analyze the results with SPSS 17.0 software package. RESULTS: Twist1 siRNA transfection reversed EMT phenotype of CAL27/CDDP. The expression of E-cadherin was up-regulated and the expression of Vimentin, Twist1 and Slug was down-regulated. The motility was significantly decreased. The IC₅₀ of CAL27/CDDP decreased by 41.75%±5.10%（P<0.01). CONCLUSIONS: Twist1 promotes EMT and CDDP drug-resistance in TSCC.

Key words: Tongue squamous cell carcinoma, Epithelial-mesenchymal transition, Twist1, Drug resistance

中图分类号:

R739.8

刘墨, 阮毅, 李劲松, 黄洪章. Twist1调控上皮-间质转化促进舌鳞癌细胞顺铂耐药机制的研究[J]. 中国口腔颌面外科杂志, 2018, 16(1): 1-5.

LIU Mo, RUAN Yi, LI Jing-song, HUANG Hong-zhang. Study on Twist1 promoting epithelial-mesenchymal transition and drug-resistance in tongue squamous cell carcinoma[J]. China J Oral Maxillofac Surg, 2018, 16(1): 1-5.

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Twist1调控上皮-间质转化促进舌鳞癌细胞顺铂耐药机制的研究

Study on Twist1 promoting epithelial-mesenchymal transition and drug-resistance in tongue squamous cell carcinoma

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