人类婆罗双树样基因4在口腔鳞状细胞癌中的表达及临床意义

摘要/Abstract

摘要： 目的: 从mRNA水平和蛋白质水平研究人类婆罗双树样基因4（SALL4）在口腔鳞状细胞癌（oral squamous cell carcinomas,OSCC）组织中的表达,分析其与口腔鳞状细胞癌的临床病理参数之间的关系,探讨其临床意义。方法: 采用实时定量PCR、Western免疫印迹及免疫组织化学方法分别检测SALL4 mRNA及蛋白在50例OSCC标本组织及癌旁正常组织中的表达,应用SPSS17.0软件包对数据进行统计学分析。结果: 实时定量PCR结果显示,SALL4 mRNA在口腔鳞状细胞癌组织中的表达量显著高于癌旁正常组织（P<0.05）;Western免疫印迹及免疫组织化学结果同样证实,SALL4在口腔鳞状细胞癌组织中的蛋白表达显著高于癌旁正常组织（P<0.05）。免疫组织化学结果显示,SALL4蛋白阳性表达定位于细胞核上,呈棕黄色颗粒状,而且 SALL4蛋白的表达与OSCC患者的肿瘤分期（P<0.05）以及有无淋巴结转移（P<0.05）相关。结论: SALL4可以作为一种具有良好敏感性和特异性的辅助指标,在口腔鳞状细胞癌的临床病理诊断和鉴别诊断中具有重要作用。而且,SALL4可能在口腔鳞状细胞癌的转移过程中发挥重要作用,但其具体生物学作用和调控机制有待进一步研究。

关键词: SALL4, 口腔鳞状细胞癌, 肿瘤干细胞

Abstract: PURPOSE: To investigate the expression and clinical significance of Sal-like protein 4 (SALL4) in oral squamous cell carcinomas (OSCC). METHODS: Real-time PCR, Western blotting and immunohistochemistry were used to analyze the expressions levels of SALL4 mRNA and protein in 50 OSCC tissue specimens in comparison with their adjacent normal tissue specimens. SPSS17.0 software package was used to identify correlations between SALL4 expression and the clinicopathological features in OSCC. RESULTS: Expression of SALL4 was upregulated in OSCC tissue at both mRNA and protein levels compared with the adjacent normal tissue (P<0.05). Immunohistochemistry detected positive nucleus staining of SALL4 protein. Expression of SALL4 was significantly correlated with OSCC staging and lymph node metastasis (both P<0.05). CONCLUSIONS: SALL4 may be an extremely useful diagnostic marker for OSCC. Furthermore, SALL4 may play an important role in metastasis of OSCC; however, the underlying molecular mechanism needs to be further investigated.

Key words: SALL4, Oral squamous cell carcinomas, Cancer stem cell

中图分类号:

R739.8

刘思强, 孙娜, 张风河. 人类婆罗双树样基因4在口腔鳞状细胞癌中的表达及临床意义[J]. 中国口腔颌面外科杂志, 2016, 14(5): 440-444.

LIU Si-qiang, SUN Na, ZHANG Feng-he. Expression and clinical significance of SALL4 in oral squamous cell carcinoma[J]. China J Oral Maxillofac Surg, 2016, 14(5): 440-444.

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