大鼠触须运动功能恢复记录方法的探讨

摘要/Abstract

摘要： 目的: 介绍一种定量记录面神经损伤修复后触须运动功能恢复程度和过程的方法。方法: 32只大鼠经适应性训练3周后,随机分为面神经干钳夹损伤组（A组）、面神经干切断吻合组（B组）、面神经干假手术组（C组）、面神经干切断不修复组（D组）;限制大鼠身体活动,标记双侧B排3根触须,视频记录术前、术后2、4、8、12周触须活动5 min;选择头颅位置恒定、触须摆动最活跃的5 s片断,用Proanalyst运动分析软件、SPSS 21.0软件包,分别计算摆动幅度、频率,双尾配对和独立样本t检验。结果: 术前双侧触须摆动幅度、频率的差异无统计学意义。A组术后2周术侧摆动幅度达到正常侧的77%,12周时摆动幅度恢复至正常侧的96%。B组术后2周术侧触须仅出现轻微颤动,4周时摆动幅度达到正常侧的40%,12周时摆动幅度恢复至正常侧的77%。术后4周起,A组、B组两侧触须摆动频率的差异无统计学意义。C组术后2周术侧触须恢复正常摆动。D组在观察期内术侧触须无功能恢复迹象。结论: 限制身体活动时,双侧触须对称、同步摆动;视频技术与身体限制措施、运动分析软件相结合,可以满足面神经损伤修复后功能评价的需要。

关键词: 大鼠, 面神经, 触须, 视频记录, 摆动

Abstract: PURPOSE: To introduce a simple, objective and quantifiable videographic method for tracking the process and extent of vibrissal motor recovery following facial nerve injury. METHODS: Thirty two adult female Sprague-Dawley rats were subject to adaptive training for 3 weeks and then they were randomized into 4 groups (n=8 in each group): main trunk experimental crush injury (group A), main trunk transection and end to end suture (group B), main trunk sham surgery control (group C) and main trunk transection denervation control (group D). Whisking recovery was measured on a scheduled basis for 2, 4, 8 and 12 weeks. The rats underwent a 5-min videographic recording session during which tagged vibrissae were continuously monitored. The video sequences were slowly reviewed and 5 s sequence fragments containing the most vigorous whisking were analyzed offline using Proanalyst motion analysis software. The selection criteria used were stable position of the head, frequency of whisking, and amplitude of vibrissal protraction. The data were analyzed using SPSS 21.0 software package. RESULTS: There was no significant difference in any preoperative whisking parameters between the manipulated side and intact side. In group A, 2 weeks after surgery, the mean whisking amplitude was approximately 77% of its value on the intact sides, and recovered approximately 96% within 12 weeks. In group B, at 10-14 days, the vibrissae "rose" again to the level of the mouth and acquired a posterior orientation with only slight tremor-like movements; by the 4 week, the rats had approximately 40% of recovery of whisking amplitude compared to the intact sides; 12 weeks postoperatively, the whisking amplitude reached 77%. Since the 4 week, group A and B had similar whisking frequency in both sides. Fourteen days postoperatively, all animals in group C exhibited normal whisking function compared with the preoperative vigorous whisking. Animals in group D had no recovery of whisking function during the study. CONCLUSIONS: Observations on body restrained rats showed that majority of free whisking on both sides is synchronous and symmetric, which allows us to compare vibrissal motor data between intact and manipulated side after facial nerve injury. The videographic technique, when used in conjunction with body restrained procedures and motion analysis software, may be an objective tool to evaluate motor recovery from facial nerve manipulation in the rat model.

Key words: Rat, Facial nerve, Vibrissa, Vediographic recording, Whisking

中图分类号:

R745.1

程嘉, 吴姝, 费亦凡, 刘渊, 黄建涛, 赵云富. 大鼠触须运动功能恢复记录方法的探讨[J]. 中国口腔颌面外科杂志, 2016, 14(5): 404-408.

CHENG Jia, WU Shu, FEI Yi-fan, LIU Yuan, HUANG Jian-tao, ZHAO Yun-fu. Methods for recording vibrissal motor recovery in rats[J]. China J Oral Maxillofac Surg, 2016, 14(5): 404-408.

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