miR-214在口腔鳞状细胞癌中的表达及功能研究

中国口腔颌面外科杂志 ›› 2016, Vol. 14 ›› Issue (2): 97-105.

• 论著 • 下一篇

miR-214在口腔鳞状细胞癌中的表达及功能研究

吴越^{1, 2}, 王成^{1, 2}, 谢楠^{1, 3}, 庄泽航^{1, 2}, 刘习强^{1, 2}, 侯劲松^{1, 2}, 黄洪章^{1, 2}

1. 中山大学光华口腔医学院·附属口腔医院口腔颌面外科,广东广州 510055;
2. 广东省口腔医学重点实验室,广东广州 510055;
3. 中山大学光华口腔医学院·附属口腔医院口腔病理科,广东广州 510055

收稿日期:2015-11-19 出版日期:2016-03-20 发布日期:2016-04-06
通讯作者: 黄洪章,Tel:020-83860121,E-mail:huanghongzhang@tom.com
作者简介:吴越（1989-）,女,硕士,E-mail:wuhuaguo1989@126.com
基金资助:
国家自然科学基金（81272949,81572661,81202136）; 高等学校博士学科点专项科研基金（20120171120068）; 广州市珠江科技新星专项（2014J2200045）; 中央高校基本科研业务费专项资金中山大学青年教师培育计划（11ykpy47）

Expression and functional role of miR-214 in oral squamous cell carcinoma

WU Yue^{1, 2}, WANG Cheng^{1, 2}, XIE Nan^{1, 3}, ZHUANG Ze-hang^{1, 2}, LIU Xi-qiang^{1, 2}, HOU Jin-song^{1, 2}, HUANG Hong-zhang^{1, 2}

1.Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat -sen University. Guangzhou 510055; 2.Guangdong Provincial Key Laboratory. Guangzhou 510055; 3.Department of Oral Pathology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat -sen University. Guangzhou 510055, Guangdong Province, China

Received:2015-11-19 Online:2016-03-20 Published:2016-04-06

摘要/Abstract

摘要： 目的:探讨miR-214在口腔鳞状细胞癌中的表达及其对口腔鳞癌细胞侵袭、迁移、增殖和凋亡能力的影响。方法:实时荧光定量RT-PCR检测31例口腔鳞状细胞癌组织和7株口腔鳞状细胞癌细胞系中miR-214表达水平,分析其与患者临床病理参数的相关性;评估miR-214在7株细胞系中的基础表达水平,分别选择miR-214高、低表达的细胞系进行后续研究。对选择的细胞系分别转染miR-214 mimics和inhibitors,采用划痕实验、Transwell实验、流式细胞仪分别检测转染后细胞侵袭、迁移、增殖和凋亡能力的变化。采用SPSS19.0软件包对数据进行统计学处理。结果:在口腔鳞状细胞癌中,miR-214的表达与患者颈淋巴结转移情况和临床分期呈正相关（P<0.05）;转染miR-214 mimics的UM1细胞侵袭、迁移能力显著高于对照组（P<0.05）,转染miR-214 inhibitors的SCC9细胞侵袭、迁移能力显著低于对照组（P<0.05）;转染miR-214 mimics的UM1细胞周期阻滞在G1期,凋亡水平无明显变化,转染miR-214 inhibitors的SCC9细胞凋亡水平和细胞周期无明显变化。结论:miR-214与口腔鳞状细胞癌恶性表型相关,可增强口腔鳞状细胞癌细胞的侵袭和迁移能力。

关键词: miR-214, 口腔鳞癌, 侵袭, 迁移

Abstract: PURPOSE: To evaluate the expression of miR-214 and investigate its functional role in oral squamous cell carcinoma (OSCC). METHODS: RT-PCR was performed to detect miR-214 expression levels in 31 OSCC tissues and 7 OSCC cell lines. The correlation of miR-214 expression with the clinicopathologic features of the patients was analyzed with Chi-square test. Then, OSCC cells were transfected with miR-214 mimics and inhibitors. Wound healing assay and Transwell assay were performed to determine cell migratory and invasive abilities. Flow cytometry was used to assess cell cycle and apoptosis. SPSS19.0 software package was used for statistical analysis. RESULTS: In 31 OSCC patients, the expression of miR-214 positively correlated with lymph node metastasis and clinical stage (P<0.05). In vitro, the invasion and migration abilities were substantially enhanced in UM1 cells (P<0.05) transfected with miR-214 mimics and decreased (P<0.05) in SCC9 cells transfected with miR-214 inhibitors. Cell cycle was arrested in G1 stage but the apoptosis was not significantly increased in UM1 cells treated with miR-214 mimics. However, there were no obvious changes on cell cycle and apoptosis in SCC9 cells treated with miR-214 inhibitors. CONCLUSIONS: miR-214 is correlated with cervical lymph node metastasis and clinical stage in OSCC. It may act as an oncomiRNA to promote invasion and migration in OSCC.

Key words: miR-214, Oral squamous cell carcinoma, Invasion, Migration

中图分类号:

R739.8

吴越, 王成, 谢楠, 庄泽航, 刘习强, 侯劲松, 黄洪章. miR-214在口腔鳞状细胞癌中的表达及功能研究[J]. 中国口腔颌面外科杂志, 2016, 14(2): 97-105.

WU Yue, WANG Cheng, XIE Nan, ZHUANG Ze-hang, LIU Xi-qiang, HOU Jin-song, HUANG Hong-zhang. Expression and functional role of miR-214 in oral squamous cell carcinoma[J]. China J Oral Maxillofac Surg, 2016, 14(2): 97-105.

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miR-214在口腔鳞状细胞癌中的表达及功能研究

Expression and functional role of miR-214 in oral squamous cell carcinoma

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