hOPG基因转染对种植体周围骨再生的影响

中国口腔颌面外科杂志 ›› 2016, Vol. 14 ›› Issue (1): 1-7.

• 论著 • 下一篇

hOPG基因转染对种植体周围骨再生的影响

李佳洋，尹国柱，孙玥，陈贵征，纪焕中，罗恩

四川大学华西口腔医学院·口腔疾病研究国家重点实验室,四川成都 610041

收稿日期:2015-05-06 出版日期:2016-01-20 发布日期:2016-02-01
通讯作者: 罗恩,Tel:028-85502334, E-mail:luoen521125@sina.com
作者简介:李佳洋（1993-）,女,口腔医学8年制学生,E-mail:1553205723@qq.com
基金资助:
四川省科技厅科技支撑计划（2015SZ0127,2014SZ0019-4）

Enhanced osteogenesis in peri-implant through local hOPG transfection

LI Jia-yang, YIN Guo-zhu, SUN Yue, CHEN Gui-zhen, JI Huan-zhong, LUO En

West China Hospital of Stomatology, State Key Laboratory of Oral Disease, Sichuan University. Chengdu 610041, Sichuan Province, China

Received:2015-05-06 Online:2016-01-20 Published:2016-02-01

摘要/Abstract

摘要： 目的研究局部稳定表达OPG在种植体周围骨再生修复过程中的作用。方法将66只SD大鼠随机分为3组,A组种植体周围注射腺病毒Ad5-OPG-EGFP,B组种植体周围注射腺病毒Ad5-EGFP,C组种植体周围注射相同体积的PBS。在植入手术后1周、4周处死动物,行RT-PCR、组织学观察和Micro-CT扫描等检测,采用SPSS15.0软件包对相关数据进行t检验。结果实验组hOPG表达量显著高于对照组(P<0.05),证实重组腺病毒载体Ad5-OPG-EGFP建模成功,可顺利转染到骨组织中并稳定表达。植入术后4周,实验组和对照B组和C组大鼠均有新骨形成、包绕种植体。A组骨面积百分比(%BIC)、骨结合百分比(%BA)、标化后骨体积(BV/TV)、骨结合率(%OI)、单位体积内骨小梁数量(Tb.N)、单位体积内骨小梁厚度(Tb.Th)、骨小梁连接密度(Conn.D)均显著高于B组和C组(P<0.05),单位体积内骨小梁间隔(Tb.Sp)则显著低于B组和C组。结论 hOPG基因转染可在种植体周围稳定表达OPG,加快骨质再生,使种植体骨结合率增加。

关键词: 种植体, 腺病毒, 骨保护素, 骨结合

Abstract: PURPOSE : To study the role of local stable expression of OPG in peri-implant in bone regeneration during repair process. METHODS : Sixty-six SD rats were divided into A, B and C group randomly. In group A, adenovirus-hOPG-EGFP was injected around the implant; in group B, adenovirus-EGFP was injected around the implant; in group C, same volume of PBS was injected in the periphery of implant. The animals were sacrificed 1 week, 4 weeks after operation, and the femur bone containing the implant was collected. Micro-CT, H-E staining, toluidine blue staining were used to observe new bone formation. The data was analyzed with SPSS 15.0 software package. RESULTS : A successful vector of reconstructed adenovirus- OPG-GFP as gene therapy was established. In vitro the GFP was successfully expressed in the BMSCs of SD rats. At 4 weeks after implant surgery, osteointegration was observed in all groups. However, % BIC, % bone area, BV/TV, % OI, Tb.N, Tb.Th, Conn.D in the peri-implant area in group A significantly increased compared with the control group (B and C)(P<0.05). Tb.Sp was also significantly less in experimental group (A) than that in control group (B and C). CONCLUSIONS : Local hOPG transfection can be successfully conducted, and the stable expression of protein OPG in peri-implant area can remarkably promote osteogenesis.

Key words: Implant, Adenovirus, Osteoprotegerin, Osteointegration

中图分类号:

R782.1

李佳洋，尹国柱，孙玥，陈贵征，纪焕中，罗恩. hOPG基因转染对种植体周围骨再生的影响[J]. 中国口腔颌面外科杂志, 2016, 14(1): 1-7.

LI Jia-yang, YIN Guo-zhu, SUN Yue, CHEN Gui-zhen, JI Huan-zhong, LUO En. Enhanced osteogenesis in peri-implant through local hOPG transfection[J]. China J Oral Maxillofac Surg, 2016, 14(1): 1-7.

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hOPG基因转染对种植体周围骨再生的影响

Enhanced osteogenesis in peri-implant through local hOPG transfection

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