组织块法建立婴幼儿血管瘤动物模型的实验研究

中国口腔颌面外科杂志 ›› 2015, Vol. 13 ›› Issue (6): 486-490.

组织块法建立婴幼儿血管瘤动物模型的实验研究

彭昊，周国瑜，赵建鑫，沈玲悦，马川

上海交通大学医学院附属第九人民医院口腔颌面-头颈肿瘤科，激光美容科，上海市口腔医学重点实验室，上海 200011

收稿日期:2015-05-04 出版日期:2015-11-20 发布日期:2015-12-03
通讯作者: 周国瑜，E-mail:guoyuzhou@hotmail.com
作者简介:彭昊(1990-)，女，在读硕士研究生，E-mail:penghao46@gmail.com
基金资助:
Supported by Research Fund of Science and Technology Committee of Shanghai Municipality(12140902202).

Establishment of infantile hemangioma on nude mice with xenograft of human hemangioma tissue

PENG Hao, ZHOU Guo-yu, ZHAO Jian-xin, SHEN Ling-yue, MA Chuan.

Department of Oromaxillofacial Head and Neck oncology, Laser Cosmetology, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine;Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2015-05-04 Online:2015-11-20 Published:2015-12-03
Contact: 上海市科学技术委员会资助项目（12140902202）

摘要/Abstract

摘要： 目的：探索建立婴幼儿血管瘤动物模型的方法。方法：将16只裸鼠随机分为2组，一组每周肌注雌二醇1次，每次0.1 mg；另一组为对照组，将不同类型的婴儿颌面部血管瘤标本分别植入16只裸鼠体内，观察其生长状况，测量其体积变化，并于90 d时进行病理学检查，进行CD31、CD34、Ki67免疫组织化学染色和CD34免疫荧光检测。应用SPSS13.0软件包对数据进行统计学分析。结果：实验组移植瘤体显著大于对照组（P<0.05），其生长特点与人血管瘤相似。光镜下检测符合血管瘤增殖期的病理特点，CD31、CD34、Ki67检测呈强阳性，与裸鼠瘤周正常肌肉组织相比，差异显著（P<0.05）。结论：通过组织块移植法建立婴幼儿血管瘤模型是一种可靠的方法，为婴幼儿血管瘤的研究提供了平台。

关键词: 婴幼儿血管瘤, 动物模型, 免疫组织化学

Abstract: PURPOSE : To find a way to establish a human hemangioma model on nude mice. METHODS : Sixteen BALB/c nude mice were randomly divided into 2 groups. In one group, the mice were administered intramuscularly with 0.01 mg estradiol every week. Additionally, and another group with normal diet was as control. The specimen of human maxillofacial hemangioma was inoculated subcutaneously into 16 nude mice. The changes of hemangioma tissues were observed and harvested on the 90th day after graft, and then subjected to histopathological examination and immunohistochemical staining for CD31, CD34, Ki67. Statistical analysis was performed using SPSS 13.0 software package. RESULTS : It was found that the volume of hemangioma tissues in the experimental group increased more greatly than the control group, and the growth characteristics were similar to human hemangioma. Statistical differences were found between hemangioma tissues and surrounding normal muscle tissues in CD31, CD34, Ki67 staining. CONCLUSIONS : It is possible to establish an experimental model of human infantile hemangioma by implanting human hemangioma tissue into nude mice, which could lay a foundation for further study of human hemangioma.

Key words: Infantile hemangioma, Animal model, Immunohistochemistry

中图分类号:

R739.8

彭昊，周国瑜，赵建鑫，沈玲悦，马川. 组织块法建立婴幼儿血管瘤动物模型的实验研究[J]. 中国口腔颌面外科杂志, 2015, 13(6): 486-490.

PENG Hao, ZHOU Guo-yu, ZHAO Jian-xin, SHEN Ling-yue, MA Chuan.. Establishment of infantile hemangioma on nude mice with xenograft of human hemangioma tissue[J]. China J Oral Maxillofac Surg, 2015, 13(6): 486-490.

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