大鼠骨髓间充质细胞电离辐射敏感性的体外实验研究

中国口腔颌面外科杂志 ›› 2015, Vol. 13 ›› Issue (3): 205-210.

大鼠骨髓间充质细胞电离辐射敏感性的体外实验研究

刘忠龙¹, 代天国¹, 付水霆¹, 胡海生², 何悦¹

1.上海交通大学医学院附属第九人民医院·口腔医学院口腔颌面-头颈肿瘤科,上海市口腔医学重点实验室,
2.口腔放疗科,上海 200011

收稿日期:2014-10-14 修回日期:2015-01-24 出版日期:2015-05-20 发布日期:2015-06-18
通讯作者: 何悦,Tel:021-23271699-5656,E-mail:william5218@126.com
作者简介:刘忠龙（1988-）,男,硕士研究生,E-mail:eddielew021@gmail.com
基金资助:
国家自然科学基金（81271112）; 上海市人才发展基金（201312）; 上海交通大学晨星学者计划A类

In vitro study of the radiosensitivity of bone marrow stem cells isolated from Sprague-Dawley rats

LIU Zhong-long¹, DAI Tian-guo¹, FU Shui-ting¹, HU Hai-sheng², HE Yue¹

1.Department of Oromaxillofacial Head and Neck Oncology, Shanghai 200011, China;
2.Department of Radiotherapy, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2014-10-14 Revised:2015-01-24 Online:2015-05-20 Published:2015-06-18
Supported by:
Supported by National Natural Science Foundation of China (81271112), Development Foundation of Shanghai Municipal Human Resources and Social Security Bureau (201312), and SMC Rising Star (A) Scholar of Shanghai Jiao Tong University

摘要/Abstract

摘要： 目的研究大鼠骨髓间充质细胞（BMSCs）对体外电离辐射的敏感性。方法分离SD大鼠BMSCs并进行体外培养。第3代细胞给予体外电离辐照处理,剂量分别为0、4、8、10 Gy。CCK-8法检测细胞增殖,流式细胞技术检测细胞凋亡,实时定量荧光PCR检测辐照后24 h和7 d Casepase-3及Cyclin-D1基因的表达。采用SPSS20.0软件包对数据进行统计学分析。结果成功分离并体外培养大鼠BMSCs。电离辐照后,增殖曲线提示10 Gy剂量组较对照组生长速度快。凋亡检测提示10 Gy剂量组死亡细胞及晚期凋亡细胞比例较对照组显著升高。10 Gy剂量辐照后,Cyclin-D1基因在辐照后第24小时（P<0.01）和第7天（P<0.001）表达显著上调。Casepase-3基因在10 Gy辐照后24 h显著上调（P<0.01）。结论 BMSCs在体外对电离辐射具有一定的抵抗性。

关键词: 大鼠骨髓间充质细胞, 电离辐射, 细胞增殖, 细胞凋亡

Abstract: PURPOSE: To investigate the radiosensitivity of bone marrow stem cells (BMSCs) isolated from mice in vitro. METHODS: BMSCs was isolated from Sprague-Dawley rats and subsequently cultured in vitro. Third passage of BMSCs was exposed under ionizing radiation with various doses (0,4,8,10 Gy). Cell proliferation was detected using CCK-8 method and flow cytometry was applied to identify the cell apoptosis. Real-time PCR was used to measure the gene expression of Cyclin-D1 and Casepase-3 at 24 hour and 7 day. The difference between various treatment groups was analyzed with SPSS 20.0 software package. RESULTS: BMSCs were successfully isolated and cultured in vitro. Cell growth curve analysis indicated that BMSCs with 10 Gy treatment showed faster growth compared with the control group. Meanwhile, group with 10 Gy irradiation showed higher proportion of died cells and apoptotic cells than those treated with other doses. Significant up-regulation of Cyclin-D1 was found in group with 10 Gy irradiation at 24 hour (P<0.01) and 7 day (P<0.001).Up-regulation of Casepase-3 was only found at 24 hour (P<0.01). CONCLUSIONS: BMSCs of SD rats showed radioresistance following ionizing radiation in vitro.

Key words: Bone marrow stem cells, Ionizing radiation, Cell proliferation, Cell apoptosis

中图分类号:

Q274

刘忠龙, 代天国, 付水霆, 胡海生, 何悦. 大鼠骨髓间充质细胞电离辐射敏感性的体外实验研究[J]. 中国口腔颌面外科杂志, 2015, 13(3): 205-210.

LIU Zhong-long, DAI Tian-guo, FU Shui-ting, HU Hai-sheng, HE Yue. In vitro study of the radiosensitivity of bone marrow stem cells isolated from Sprague-Dawley rats[J]. China J Oral Maxillofac Surg, 2015, 13(3): 205-210.

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