巨噬细胞在β-TCP支架修复牙槽突裂中成骨作用的探讨

doi:10.19438/j.cjoms.2021.04.005

中国口腔颌面外科杂志 ›› 2021, Vol. 19 ›› Issue (4): 315-319.doi: 10.19438/j.cjoms.2021.04.005

巨噬细胞在β-TCP支架修复牙槽突裂中成骨作用的探讨

单宇华, 应惜裕, 张晓宇, 陈振琦

上海交通大学医学院附属第九人民医院口腔正畸科,上海交通大学口腔医学院,国家口腔医学中心, 国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海 200011

收稿日期:2021-01-19 修回日期:2021-03-14 出版日期:2021-07-20 发布日期:2021-08-05
通讯作者: 陈振琦,E-mail：orthochen@yeah.net
作者简介:单宇华（1996-）,女,在读硕士研究生,E-mail：13916646073@163.com

Study on the osteogenic potential of macrophages in alveolar cleft repaired with β-TCP scaffold

SHAN Yu-hua, YING Xi-yu, ZHANG Xiao-yu, CHEN Zhen-qi

Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2021-01-19 Revised:2021-03-14 Online:2021-07-20 Published:2021-08-05

摘要/Abstract

摘要： 目的： 评价使用3D打印β磷酸三钙（β-TCP）支架复合骨髓干细胞（bone marrow stem cell, BMSC）修复牙槽突裂的成骨效果以及巨噬细胞在成骨中的作用。方法： 将16只大鼠随机分为2组,将第1组8只大鼠双侧上颌第一磨牙拔除,形成4 mm×4 mm×3 mm的箱状骨缺损。8周后,一侧植入3D打印β-TCP复合干细胞支架,另一侧植入同样载有干细胞的空白支架。将第2组8只大鼠以相同方式造裂,两侧同时放置3D打印β-TCP复合干细胞支架,一侧注射氯膦酸盐脂质体清除巨噬细胞,另一侧注射对照脂质体。术后8周进行显微CT扫描和组织学分析,检测成骨效果。采用 SAS 8.0 软件包对数据进行统计学处理。结果： β-TCP支架移植侧BV/TV为(41.38±3.33)%,空白支架放置侧为(30.42±2.97)%,两者之间差异显著（P<0.05）。去除巨噬细胞后,BV/TV下降至(18.71±2.19)%,对照脂质体注射侧为(39.81±2.68)%,β-TCP支架放置侧和对照脂质体注射侧结果无统计学差异。结论： 3D打印β-TCP支架复合骨髓干细胞有较好的成骨效果,巨噬细胞在β-TCP介导成骨过程中起到重要作用,去除巨噬细胞阻碍新骨形成。

关键词: β磷酸三钙, 牙槽突裂, 骨免疫学, 骨替代物

Abstract: PRUPOSE: To study the osteogenic potential of 3D printed β-TCP scaffold combined with bone marrow stem cells（BMSCs） repairing alveolar cleft and the role of macrophages in osteogenesis. METHODS: Bilateral maxillary first molars were extracted in the first eight rats to form a box shaped bone defect in the size of 4 mm × 4 mm × 3 mm. 3D printed β-TCP scaffold with BMSCs was implanted on one side and control scaffold was implanted on the other side eight weeks later. In the second group, 3D printed β-TCP scaffold with BMSC was placed on both sides at the same time, and macrophages were removed on one side. Eight weeks after operation, Micro-CT scanning and histological analysis were performed to detect bone formation. SAS 8.0 software was performed for statistical analysis. RESULTS: BV/TV ratio was (41.38±3.334)% in β-TCP group and (30.42±2.964)% in the control group (P<0.05). BV/TV ratio was (18.71±2.193)% in macrophage-depletion group and (39.81%±2.678)% in the control group. There was no significant difference between the two groups with the same β-TCP side. CONCLUSIONS: BMSCs combined with 3D printed β-TCP scaffold has satisfying osteogenic ability, and macrophages play an important role in β-TCP mediated osteogenesis. The depletion of macrophages hinders new bone formation.

Key words: β-tricalcium phosphate, Alveolar cleft, Osteoimmunology, Bone substitute

中图分类号:

R782.2

单宇华, 应惜裕, 张晓宇, 陈振琦. 巨噬细胞在β-TCP支架修复牙槽突裂中成骨作用的探讨[J]. 中国口腔颌面外科杂志, 2021, 19(4): 315-319.

SHAN Yu-hua, YING Xi-yu, ZHANG Xiao-yu, CHEN Zhen-qi. Study on the osteogenic potential of macrophages in alveolar cleft repaired with β-TCP scaffold[J]. China J Oral Maxillofac Surg, 2021, 19(4): 315-319.

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巨噬细胞在β-TCP支架修复牙槽突裂中成骨作用的探讨

Study on the osteogenic potential of macrophages in alveolar cleft repaired with β-TCP scaffold

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