中国口腔颌面外科杂志

中国口腔颌面外科杂志 ›› 2014, Vol. 12 ›› Issue (3): 193-197.

• 基础研究 •    下一篇

口腔鳞癌相关血清多肽的筛选和鉴定

刘莹,谈亦然,杨丞喆,朱东旺,钟来平,张陈平,张志愿   

  1. 上海交通大学医学院附属第九人民医院·口腔医学院 口腔颌面-头颈肿瘤科, 上海市口腔医学重点实验室,上海 200011
  • 收稿日期:2013-09-16 出版日期:2014-05-10 发布日期:2014-07-15
  • 通讯作者: 钟来平,Tel:021-23271699-5160,E-mail:zhonglaiping@163.com E-mail:allyliuying@sjtu.edu.cn
  • 作者简介:刘莹(1988-),女,在读硕士研究生
  • 基金资助:
    国家自然科学基金(81272979);上海市科学技术委员会基金(1052nm04700,13QH1401700);上海交通大学医工交叉基金(YG2010MS49)

Serum peptide analysis in oral squamous cell carcinoma

LIU Ying, TAN Yi-ran, YANG Cheng-zhe, ZHU Dong-wang, ZHONG Lai-ping, ZHANG Chen-ping, ZHANG Zhi-yuan   

  1. Department of Oromaxillofacial Head and Neck Oncology, Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China
  • Received:2013-09-16 Online:2014-05-10 Published:2014-07-15

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摘要： 目的:探讨口腔鳞癌不同发展阶段的差异血清多肽谱。方法:采用基质辅助激光解析飞行时间质谱结合弱阳离子纳米磁珠,检测120例健康正常人、37例早期口腔鳞癌患者、129例晚期口腔鳞癌患者血清多肽指纹图谱,应用ClinProtToolsTM 2.1软件对检测结果进行分析,建立差异多肽的鉴别诊断模型,对模型中的差异多肽进行质谱分析,以IPI数据库检索确定相应蛋白质名称。结果:在3组间的两两比较中,共筛选出385个差异多肽,初步建立口腔鳞癌与健康正常人及口腔鳞癌早、晚期的鉴别诊断模型。早期口腔鳞癌与健康正常人之间的鉴别诊断模型包括5个差异多肽峰,分别是FGA、ITIH4、PKM2、SCARA5和SDPR,诊断敏感度和特异度为82.4%和98.5%;晚期口腔鳞癌与早期口腔鳞癌之间的鉴别诊断模型包括4个差异多肽峰,诊断敏感度和特异度为98.5%和88.2%,分别是FGA、PKM2、ITIH4和ALB;晚期口腔鳞癌与健康正常人之间的鉴别诊断模型包括5个差异多肽峰,其中3个为SERPINA1、TTR和SDPR,诊断敏感度和特异度为89.4%和85.1%。结论:采用多肽组学方法,鉴定了部分口腔鳞癌不同发展阶段的差异多肽,为进一步开展血清多肽验证和差异蛋白质的机制研究奠定了基础。

关键词: 口腔鳞癌, 基质解析飞行时间质谱, 纳米磁珠, 血清多肽

Abstract: PURPOSE: To explore serum peptide profiles in patients with oral squamous cell carcinoma (OSCC) at early and late clinical stages. METHODS: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) combined with weak cation magnetic nano-beads (WCX) were used to detect serum peptide profiles in 120 healthy persons, 37 OSCC patients at early clinical stage and 129 OSCC patients at late clinical stage. ClinprotTools 2.1TM software was used to analyze different serum peptide peaks and establish differential diagnosis model. Potential significant peaks were identified by searching IPI database. RESULTS: A total of 385 differential serum peptide peaks were found among healthy persons, early stage OSCC patients and late stage OSCC patients. Based on the differential diagnosis model, five peptide peaks were identified as FGA, ITIH4, PKM2, SCARA5 and SDPR, which could separate OSCC patients at early clinical stage from healthy person with sensitivity of 82.4% and specificity of 98.5%; four peptide peaks were identified as FGA, PKM2, ITIH4 and ALB, which could separate OSCC patients at early clinical stage from those at late clinical stage with sensitivity of 98.5% and specificity of 88.2%; five peptide peaks could be used to separate OSCC patients at late clinical stage from healthy persons with sensitivity of 89.4% and specificity of 85.1%, three of them were identified as SERPINA1,TTR and SDPR. CONCLUSION: From these results, potential differential serum peptides are identified to separate OSCC patients from healthy persons. These potential serum biomarkers should be further validated with mechanism studies before application in clinic. Supported by National Natural Science Foundation of China (81272979), Research Fund of Science and Technology Committee of Shanghai Municipality (1052nm04700 and 13QH1401700), and Medicine and Engineering Cross Research Fund of Shanghai Jiao Tong University (YG2010MS49).

Key words: Oral squamous cell carcinoma, Matrix-assisted laser desorption time of flight mass, Magnetic nano-beads, Serum peptide

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