中国口腔颌面外科杂志 ›› 2025, Vol. 23 ›› Issue (3): 244-252.doi: 10.19438/j.cjoms.2025.03.006

• 论著 • 上一篇    下一篇

60例口腔鳞癌p53染色模式与TP53突变状态的相关性分析

刘瑜1,*, 缪可言1,*, 胡宇华2, 吴越1, 冯冠英1, 夏荣辉2#, 杨溪1#   

  1. 1.上海交通大学医学院附属第九人民医院 口腔颌面-头颈肿瘤科,2.口腔病理科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011
  • 收稿日期:2025-02-06 修回日期:2025-02-27 出版日期:2025-05-20 发布日期:2025-06-05
  • 通讯作者: 夏荣辉,E-mail: xrh335@163.com;杨溪,E-mail: yangxi16@163.com。#共同通信作者
  • 作者简介:刘瑜(1998-),女,在读硕士研究生,E-mail: lscrly6389@sjtu.edu.cn;缪可言(2000-),女,在读硕士研究生,E-mail: miaokeyan@sjtu.edu.cn。*并列第一作者
  • 基金资助:
    国家自然科学基金(82072980,82272831)

Correlation between p53 staining and TP53 mutation status in 60 patients with oral squamous cell carcinoma

Liu Yu1, Miao Keyan1, Hu Yuhua2, Wu Yue1, Feng Guanying1, Xia Ronghui2, Yang Xi1   

  1. 1. Department of Oromaxillofacial Head and Neck Oncology, 2. Department of Oral Pathology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology. Shanghai 200011, China
  • Received:2025-02-06 Revised:2025-02-27 Online:2025-05-20 Published:2025-06-05

摘要: 目的:探讨口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中p53染色模式与TP53突变状态之间的相关性,以及与临床病理特征之间的联系。方法:使用全外显子组测序和p53抗体(MX008 和 YN01766m)免疫组织化学染色分析60例OSCC患者的组织样本。将TP53突变状态和p53染色模式(缺失型[Null]、过表达[OE]和野生型[WT])与临床病理特征进行比较,评估p53染色模式与TP53突变状态之间的相关性。采用GraphPad Prism 8软件对数据进行统计学分析。结果:TP53是OSCC中最常见的突变基因,该突变与较高的pTNM分期、DOI和PNI显著相关,并在公共数据库中展现出与不良预后的相关性。缺失模式检测功能失活(LOF)突变的灵敏度最高,而过表达模式可预测功能获得(GOF)突变,在患者分层方面具有临床价值。2种抗体在检测和匹配TP53突变状态方面表现相似。结论:TP53在OSCC中最常发生突变,其突变与不良临床病理特征和不良预后有关。p53染色对LOF突变敏感,有望成为GOF突变的生物标志物,为患者分层和靶向治疗提供一种经济有效的方法。

关键词: 口腔鳞状细胞癌, TP53突变, p53染色模式, 功能失活突变, 功能获得突变, 临床病理特征

Abstract: PURPOSE: This study aimed to investigate the correlation between p53 staining patterns and TP53 mutation status in oral squamous cell carcinoma(OSCC), as well as their associations with clinicopathologic features. METHODS: Tissue samples from 60 OSCC patients were analyzed using whole-exome sequencing and immunohistochemical staining with p53 antibodies (MX008 and YN01766m). TP53 mutation status and p53 staining patterns (Null, overexpression [OE], and wild type[WT]) were compared with clinicopathologic features. Correlations between p53 staining patterns and TP53 mutation status were evaluated. GraphPad Prism 8 software package was used for data analysis. RESULTS: TP53 was the most frequently mutated gene in OSCC, with mutations significantly associated with higher pTNM stage, DOI, and PNI, and correlated with poor prognosis in public datasets. The Null pattern exhibited the highest sensitivity in detecting loss-of-function (LOF) mutations, while OE pattern predicted gain-of-function (GOF) mutations and showed clinical value for patient stratification. Both antibodies had similar performance in detecting and matching TP53 mutation status. CONCLUSION: TP53 is the most common mutation in OSCC,and its mutation is associated with adverse clinicopathological features and poor prognosis. p53 staining is sensitive to LOF mutations and is expected to be a biomarker of GOF mutations, providing a cost-effective method for patient stratification and targeted therapy.

Key words: Oral squamous cell carcinoma, TP53 mutation, p53 staining pattern, LOF mutation, GOF mutation, Clinicopathologic features

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