电压依赖性阴离子通道蛋白1在口腔鳞癌中的表达、功能及临床意义

doi:10.19438/j.cjoms.2025.03.004

中国口腔颌面外科杂志 ›› 2025, Vol. 23 ›› Issue (3): 228-235.doi: 10.19438/j.cjoms.2025.03.004

电压依赖性阴离子通道蛋白1在口腔鳞癌中的表达、功能及临床意义

许成辉, 姚艳丽^#, 孙树洋^#

上海交通大学医学院附属第九人民医院口腔颌面-头颈肿瘤科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011

收稿日期:2025-01-22 修回日期:2025-03-12 出版日期:2025-05-20 发布日期:2025-06-05
通讯作者: 孙树洋,E-mail: sunshuyang@sjtu.edu.cn;姚艳丽,E-mail: yaoyanli@shsmu.edu.cn。^#共同通信作者
作者简介:许成辉(1999-),男,硕士研究生,E-mail: xuch1180@163.com
基金资助:
国家自然科学基金重点项目(82030085);国家重点研发计划(2023YFC2506403);国家自然科学基金(82202916)

Expression, function and clinical significance of voltage-dependent anion channel 1 in oral squamous cell carcinoma

Xu Chenghui, Yao Yanli, Sun Shuyang

Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology. Shanghai 200011, China

Received:2025-01-22 Revised:2025-03-12 Online:2025-05-20 Published:2025-06-05

摘要/Abstract

摘要： 目的：探讨电压依赖性阴离子通道蛋白1（voltage-dependent anion channel 1,VDAC1）在口腔鳞状细胞癌（oral squamous cell carcinoma,OSCC）中的表达水平,分析其在OSCC进展中的潜在功能及临床意义。方法：使用UALCAN数据平台,分析VDAC1在头颈鳞癌中的表达水平及其与患者预后的相关性,筛选与VDAC1表达相关的基因,分析其在头颈鳞癌中的表达水平与预后相关性。免疫组织化学染色检测VDAC1在OSCC组织中的表达水平;VDAC1抑制剂处理OSCC后,观察其对细胞增殖、迁移能力的影响。采用GraphPad Prism 10.0软件包对数据进行统计学分析。结果：VDAC1在头颈鳞癌组织中高表达,且与淋巴结转移状态显著相关;高表达的VDAC1与头颈鳞癌患者总生存期和无复发生存期显著降低相关。VDAC1的高表达与基因HSPA4、PPP2CA、NPM1和CDC23显著相关,并在头颈鳞癌中呈现高表达,且与较差的总生存期密切相关。VDAC1在OSCC组织中的表达显著高于正常组织,抑制VDAC1可显著降低OSCC增殖及迁移。结论：VDAC1在OSCC发生、发展中起着关键作用,其高表达与肿瘤的侵袭性和患者的不良预后显著相关,并与HSPA4、PPP2CA、NPM1和CDC23等基因协同作用,参与调控OSCC恶性进展。

关键词: VDAC1, 口腔鳞癌, 增殖, 迁移, 预后标志物

Abstract: PURPOSE: This study aimed to investigate the expression level of voltage-dependent anion channel 1 (VDAC1) in oral squamous cell carcinoma (OSCC) and analyze its potential role in tumor progression as well as its clinical significance. METHODS: The expression level and prognostic relevance of VDAC1 in head and neck squamous cell carcinoma (HNSCC) were analyzed using UALCAN database. Genes associated with VDAC1 expression were identified using the database, and their expression levels and prognostic significance in HNSCC were analyzed. Immunohistochemical (IHC) staining was performed to evaluate VDAC1 expression in OSCC tissues. OSCC cells were treated with a VDAC1 inhibitor to observe its effects on cell proliferation and migration. GraphPad Prism 10.0 software package was used for data analysis. RESULTS: Analysis of the database revealed that VDAC1 was overexpressed in HNSCC tissues and was strongly associated with lymph node metastasis. High VDAC1 expression was correlated with significantly poorer overall survival and recurrence free survival in HNSCC patients. Additionally, VDAC1 expression was significantly correlated with several genes, including heat shock protein A4 (HSPA4), protein phosphatase 2 catalytic subunit alpha (PPP2CA), nucleophosmin 1 (NPM1), and cell division cycle protein 23(CDC23). These genes were also highly expressed in HNSCC and were strongly associated with poorer OS in patients. IHC analysis further confirmed that VDAC1 expression was markedly elevated in OSCC tissues compared to normal tissues. Inhibition of VDAC1 significantly suppressed the proliferation and migration of OSCC cells, highlighting its pivotal role in tumor progression. CONCLUSIONS: VDAC1 plays a critical role in the progression of OSCC. Its high expression is closely associated with tumor aggressiveness and poor prognosis, suggesting that VDAC1 may serve as both a prognostic biomarker and a potential therapeutic target for OSCC. This study further reveals that VDAC1 may regulate tumor progression through its interaction with genes such as HSPA4, PPP2CA, NPM1, and CDC23, providing important insights into the pathogenesis of OSCC and the basis for precision therapy.

Key words: VDAC1, Oral squamous cell carcinoma, Proliferation, Migration, Prognostic biomarker

中图分类号:

R739.8

许成辉, 姚艳丽, 孙树洋. 电压依赖性阴离子通道蛋白1在口腔鳞癌中的表达、功能及临床意义[J]. 中国口腔颌面外科杂志, 2025, 23(3): 228-235.

Xu Chenghui, Yao Yanli, Sun Shuyang. Expression, function and clinical significance of voltage-dependent anion channel 1 in oral squamous cell carcinoma[J]. China J Oral Maxillofac Surg, 2025, 23(3): 228-235.

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电压依赖性阴离子通道蛋白1在口腔鳞癌中的表达、功能及临床意义

Expression, function and clinical significance of voltage-dependent anion channel 1 in oral squamous cell carcinoma

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