Piezo1在静脉畸形组织和细胞中的表达及意义

doi:10.19438/j.cjoms.2025.03.003

中国口腔颌面外科杂志 ›› 2025, Vol. 23 ›› Issue (3): 221-227.doi: 10.19438/j.cjoms.2025.03.003

Piezo1在静脉畸形组织和细胞中的表达及意义

麦迪娜·伊马木^1,2, 周昱川^1,2,*, 穆扎帕尔·木合塔尔^1,2, 龚忠诚^1,2,*

1.新疆医科大学第一附属医院（附属口腔医院）口腔颌面肿瘤外科,新疆乌鲁木齐 830054;
2.新疆维吾尔自治区口腔医学研究所,新疆乌鲁木齐 830054

收稿日期:2024-11-19 修回日期:2025-01-21 出版日期:2025-05-20 发布日期:2025-06-05
通讯作者: 龚忠诚,E-mail: gump0904@aliyun.com;周昱川,E-mail: 843806258@qq.com。^*共同通信作者
作者简介:麦迪娜·伊马木（1998-）,女,在读硕士研究生,E-mail: 3497095393@qq.com
基金资助:
新疆维吾尔自治区自然科学基金(2022D01C252);新疆维吾尔自治区研究生创新项目(XJ2024G194)

Study of Piezo1 expression in venous malformation tissues and HUVECs

Maidina Imam^1,2, Zhou Yuchuan^1,2, Muzafar Muhetar^1,2, Gong Zhongcheng^1,2

1. Department of Oral and Maxillofacial Oncology Surgery, First Afiliated Hospital of Xinjiang Medical University Affiliated Stomatological Hospital. Urumqi 830054;
2. Xinjiang Uygur Autonomous Region Institute of Stomatology. Urumqi 830054, Xinjiang Uygur Autonomous Region, China

Received:2024-11-19 Revised:2025-01-21 Online:2025-05-20 Published:2025-06-05

摘要/Abstract

摘要： 目的：探讨Piezo1在静脉畸形组织和细胞中的表达及意义。方法：获取确诊为静脉畸形的患者蜡块标本,采用免疫组织化学技术检测静脉畸形组织及正常静脉组织标本中Piezo1、TRPV4、P2X7、CD31、α-SMA等抗体的表达水平;在人脐静脉内皮细胞（HUVECs）中过表达TIE2-L914F和TIE2-WT基因,构建静脉畸形细胞模型和野生型细胞模型,以HUVECs为对照组,采用实时反转录定量PCR（RT-qPCR）、蛋白免疫印迹试验（Western blot）及酶联免疫吸附试验（ELISA）等方法,分别检测各组细胞中Piezo1、TRPV4及P2X7 mRNA及蛋白的表达水平。采用SPSS 27.0软件包对数据进行统计学分析。结果：免疫组织化学染色结果显示,正常静脉组织及静脉畸形组织中Piezo1表达均为阳性,TRPV4及P2X7表达均为阴性。Western blot 结果显示,3组细胞的Piezo1蛋白表达水平有显著差异,TIE2-L914F 组的Piezo1蛋白水平显著下调（P<0.01）,3组细胞的TRPV4及P2X7蛋白表达水平无显著差异（P>0.05）。ELISA结果显示,Piezo1在静脉畸形组中的表达显著低于正常细胞组（P<0.0001）,3组细胞的TRPV4表达无显著差异（P>0.05）,3组细胞的P2X7表达水平无显著差异（P>0.05）。RT-qPCR结果显示,TIE2-L914F 组的Piezo1 mRNA水平较TIE2-WT 组和HUVEC 组显著下调（P<0.001）。结论：Piezo1在静脉畸形组织中较正常组织表达降低,提示Piezo1参与血管的发育及重塑。

关键词: 静脉畸形, 人脐静脉内皮细胞, 机械敏感性阳离子通道, Piezo1

Abstract: PURPOSE: To investigate the expression of Piezo1 in venous malformation(VM) tissues and human umbilical vein endothelial cells (HUVECs). METHODS: The wax samples of patients diagnosed with venous malformations were obtained, and the expression levels of antibodies Piezo1, TRPV4, P2X7, CD31 and α-SMA were detected by immunohistochemistry in the samples of venous malformations and normal veins. Overexpression of TIE2-L914F and TIE2-WT genes in HUVECs led to construction of venous malformation cell models and wild-type cell models, with HUVECs as the control group. The expression of mRNA and protein levels of Piezo1, TRPV4 and P2X7 in each group were detected by RT-qPCR, Western blot and enzyme-linked immunosorbent assay(ELISA). SPSS 27.0 software package was used for data analysis. RESULTS: Immunohistochemical results showed that Piezo1 expression was positive in both normal vein tissue and VM tissue, while TRPV4 and P2X7 expression were negative. Western blot results showed that there were significant differences in Piezo1 protein expression levels among the three groups, and the Piezo1 protein levels of the TIE2-L914F group were significantly down-regulated(P<0.01). There were no significant differences in the expression levels of TRPV4 and P2X7 proteins among the three groups(P>0.05). ELISA results showed that the expression of Piezo1 in the VM group was significantly lower than that in the normal cell group(P<0.0001), and the expression of TRPV4 in the three groups had no significant difference (P>0.05), and the expression of P2X7 in the three groups had no significant difference (P>0.05). RT-qPCR results showed that Piezo1 mRNA levels in TIE2-L914F group were significantly down-regulated compared with TIE2-WT and HUVEC group (P<0.001). CONCLUSIONS: The expression of Piezo1 in venous malformation tissues is lower than that in normal tissues, suggesting that Piezo1 is involved in the development and remodeling of blood vessels.

Key words: Venous malformation, HUVECs, Mechanosensitive ion channel, Piezo1

中图分类号:

R782.2

麦迪娜·伊马木, 周昱川, 穆扎帕尔·木合塔尔, 龚忠诚. Piezo1在静脉畸形组织和细胞中的表达及意义[J]. 中国口腔颌面外科杂志, 2025, 23(3): 221-227.

Maidina Imam, Zhou Yuchuan, Muzafar Muhetar, Gong Zhongcheng. Study of Piezo1 expression in venous malformation tissues and HUVECs[J]. China J Oral Maxillofac Surg, 2025, 23(3): 221-227.

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Piezo1在静脉畸形组织和细胞中的表达及意义

Study of Piezo1 expression in venous malformation tissues and HUVECs

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