外泌体miRNA在口腔疾病中的研究进展

doi:10.19438/j.cjoms.2022.01.017

中国口腔颌面外科杂志 ›› 2022, Vol. 20 ›› Issue (1): 88-92.doi: 10.19438/j.cjoms.2022.01.017

外泌体miRNA在口腔疾病中的研究进展

张睿鹏¹, 陈云芳², 严洪海³

1.浙江中医药大学口腔医学院,浙江杭州 310053;
2.浙江省人民医院口腔科,浙江杭州 310014;
3.浙江中医药大学附属第二医院口腔科,浙江杭州 310005

收稿日期:2020-12-04 修回日期:2021-06-07 出版日期:2022-01-20 发布日期:2022-01-20
通讯作者: 严洪海,E-mail：yhhai01@163.com
作者简介:张睿鹏（1997-）,女,在读硕士研究生E-mail：zhangruipeng@zcmu.edu.cn
基金资助:
浙江省基础公益研究项目（GF18H060022）

Research progress of exosomal miRNA in oral diseases

ZHANG Rui-peng¹, CHEN Yun-fang², YAN Hong-hai³

1. School of Stomatology, Zhejiang Chinese Medical University. Hangzhou 310053;
2. Department of Stomatology, Zhejiang Provincial People's Hospital. Hangzhou 310014;
3. Department of Stomatology, The Second Affiliated Hospital of Zhejiang Chinese Medical University. Hangzhou 310005, Zhejiang Province, China

Received:2020-12-04 Revised:2021-06-07 Online:2022-01-20 Published:2022-01-20

摘要/Abstract

摘要： 外泌体是活细胞分泌至细胞外基质的纳米级囊泡,在细胞之间的物质交换和信号转导方面发挥重要作用,能将其携带的脂质、蛋白质、核酸传递至受体细胞,调节受体细胞的生理、病理过程。微小RNA（microRNA、miRNA）作为外泌体中重要的“货物”被转运至受体细胞,调控受体细胞信号通路,从而对疾病的进展起着调节的作用。近年来,外泌体miRNA在牙周疾病、黏膜相关疾病、口腔癌等疾病中的研究日益增多。本文就外泌体miRNA在口腔疾病中的调控机制进行综述,并讨论外泌体miRNA作为生物标志物在口腔疾病诊断中的潜在前景。

关键词: 外泌体, miRNA, 口腔疾病

Abstract: Exosomes are nano-scale vesicles secreted by living cells into the extracellular matrix, and they play an important role in material exchange and signal transduction between cells. It can transfer the lipids, proteins, and nucleic acids to the recipient cells through ligand binding, phagocytosisand plasma membrane fusion, and regulate the physiological and pathological processes of the recipient cells. As an important "cargo" in exosomes, microRNAs are transported to recipient cells and participate in gene transcription and regulatory signaling pathways, thereby regulating the development of diseases. In recent years, research on exosomal miRNAs in periodontal diseases, mucosal-related diseases, oral cancer and other diseases has been increasing. This article reviewed the regulatory mechanisms of exosomal miRNAs in oral diseases, and discussed the potential prospects of exosomal miRNAs as biomarkers in the diagnosis of oral diseases.

Key words: Exosome, MicroRNA, Oral diseases

中图分类号:

张睿鹏, 陈云芳, 严洪海. 外泌体miRNA在口腔疾病中的研究进展[J]. 中国口腔颌面外科杂志, 2022, 20(1): 88-92.

ZHANG Rui-peng, CHEN Yun-fang, YAN Hong-hai. Research progress of exosomal miRNA in oral diseases[J]. China J Oral Maxillofac Surg, 2022, 20(1): 88-92.

参考文献

[1] Harding C, Heuser J, Stahl P.Endocytosis and intracellular processing of transferrin and colloidal gold-transferrin in rat reticulocytes: demonstration of a pathway for receptor shedding[J]. Eur J Cell Biol, 1984, 35(2): 256-263.
[2] McAndrews KM, Kalluri R. Mechanisms associated with biogenesis of exosomes in cancer[J]. Mol Cancer, 2019, 18(1): 52-62.
[3] Tan JL, Lau SN, Leaw B, et al.Amnion epithelial cell-derived exosomes restrict lung injury and enhance endogenous lung repair[J]. Stem Cells Transl Med, 2018, 7(2): 180-196.
[4] Brown M, Johnson LA, Leone DA, et al.Lymphatic exosomes promote dendritic cell migration along guidance cues[J]. J Cell Biol, 2018, 217(6): 2205-2221.
[5] D'Incà F, Pucillo CE. Exosomes: tiny clues for mast cell communication[J]. Front Immunol, 2015, 6: 73-74.
[6] Raposo G, Stoorvogel W.Extracellular vesicles: exosomes, microvesicles, and friends[J]. J Cell Biol, 2013, 200(4): 373-383.
[7] Hammond SM.An overview of microRNAs[J]. Adv Drug Deliv Rev, 2015, 87: 3-14.
[8] Filipowicz W, Bhattacharyya SN, Sonenberg N.Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight?[J]. Nat Rev Genet, 2008, 9(2): 102-114.
[9] Valadi H, Ekstr?m K, Bossios A, et al. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells[J]. Nat Cell Biol, 2007, 9(6): 654-659.
[10] Geng T, Song ZY, Xing JX, et al.Exosome derived from coronary serum of patients with myocardial infarction promotes angiogenesis through the miRNA-143/IGF-IR pathway[J]. Int J Nanomedicine, 2020, 15: 2647-2658.
[11] Zhu M, Zhang N, He S, et al.Exosomal miR-106a derived from gastric cancer promotes peritoneal metastasis via direct regulation of Smad7[J]. Cell Cycle, 2020, 19(10): 1200-1221.
[12] Ge Q, Zhou Y, Lu J, et al.miRNA in plasma exosome is stable under different storage conditions[J]. Molecules, 2014, 19(2): 1568-1575.
[13] Keerthikumar S, Chisanga D, Ariyaratne D, et al.ExoCarta: a web-based compendium of exosomal cargo[J]. J Mol Biol, 2016, 428(4): 688-692.
[14] Bray F, Ferlay J, Soerjomataram I, et al.Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2018, 68(6): 394-424.
[15] Rivera C.Essentials of oral cancer[J]. Int J Clin Exp Pathol, 2015, 8(9): 11884-11894.
[16] Ketabat F, Pundir M, Mohabatpour F, et al.Controlled drug delivery systems for oral cancer treatment-current status and future perspectives[J]. Pharmaceutics, 2019, 11(7): 302-330.
[17] Zhang Y, Weinberg RA.Epithelial-to-mesenchymal transition in cancer: complexity and opportunities[J]. Front Med, 2018, 12(4): 361-373.
[18] Kumar A, Deep G.Hypoxia in tumor microenvironment regulates exosome biogenesis: molecular mechanisms and translational opportunities[J]. Cancer Lett, 2020, 479: 23-30.
[19] Li L, Li C, Wang S, et al.Exosomes derived from hypoxic oral squamous cell carcinoma cells deliver miR-21 to normoxic cells to elicit a prometastatic phenotype[J]. Cancer Res, 2016, 76(7): 1770-1780.
[20] Hinshaw DC, Shevde LA.The tumor microenvironment innately modulates cancer progression[J]. Cancer Res, 2019, 79(18): 4557-4566.
[21] Tan S, Xia L, Yi P, et al.Exosomal miRNAs in tumor microenvironment[J]. J Exp Clin Cancer Res, 2020, 39(1): 67-81.
[22] Weber M, Wehrhan F, Baran C, et al.Malignant transformation of oral leukoplakia is associated with macrophage polarization[J]. J Transl Med, 2020, 18(1): 11-28.
[23] Weber M, Iliopoulos C, Moebius P, et al.Prognostic significance of macrophage polarization in early stage oral squamous cell carcinomas[J]. Oral Oncol, 2016, 52: 75-84.
[24] Cai J, Qiao B, Gao N, et al.Oral squamous cell carcinoma-derived exosomes promote M2 subtype macrophage polarization mediated by exosome-enclosed miR-29a-3p[J]. Am J Physiol Cell Physiol, 2019, 316(5): C731-C740.
[25] Li YY, Tao YW, Gao S, et al.Cancer-associated fibroblasts contribute to oral cancer cells proliferation and metastasis via exosome-mediated paracrine miR-34a-5p[J]. EBioMedicine, 2018, 36: 209-220.
[26] He L, Ping F, Fan Z, et al.Salivary exosomal miR-24-3p serves as a potential detective biomarker for oral squamous cell carcinoma screening[J]. Biomed Pharmacother, 2020, 121: 109553.
[27] Gai C, Camussi F, Broccoletti R, et al.Salivary extracellular vesicle-associated miRNAs as potential biomarkers in oral squamous cell carcinoma[J]. BMC Cancer, 2018, 18(1): 439-449.
[28] Hadavand M, Hasni S.Exosomal biomarkers in oral diseases[J]. Oral Dis, 2019, 25(1): 10-15.
[29] van der Waal I. Potentially malignant disorders of the oral and oropharyngeal mucosa; present concepts of management[J]. Oral Oncol, 2010, 46(6): 423-425.
[30] Byun JS, Hong SH, Choi JK, et al.Diagnostic profiling of salivary exosomal microRNAs in oral lichen planus patients[J]. Oral Dis, 2015, 21(8): 987-993.
[31] Ding M, Wang X, Wang C, et al.Distinct expression profile of HCMV encoded miRNAs in plasma from oral lichen planus patients[J]. J Transl Med, 2017,15(1):133-144.
[32] Voulgarelis M, Tzioufas AG.Pathogenetic mechanisms in the initiation and perpetuation of Sjogren's syndrome[J]. Nat Rev Rheumatol, 2010, 6(9): 529-537.
[33] Gallo A, Jang SI, Ong HL, et al.Targeting the Ca²+ sensor STIM1 by exosomal transfer of ebv-miR-BART13-3p is associated with Sjögren's syndrome[J]. EBioMedicine, 2016, 10: 216-226.
[34] Cortes-Troncoso J, Jang SI, Perez P, et al.T cell exosome-derived miR-142-3p impairs glandular cell function in Sjogren's syndrome[J]. JCI Insight, 2020, 5(9): e133497.
[35] Choi JW, Kim SC, Hong SH, et al.Secretable small RNAs via outer membrane vesicles in periodontal pathogens[J]. J Dent Res, 2017, 96(4): 458-466.
[36] Atsawasuwan P, Lazari P, Chen Y, et al.Secretory microRNA-29 expression in gingival crevicular fluid during orthodontic tooth movement[J]. PLoS One, 2018, 13(3): e194238.
[37] Wang M, Yuan Y, Wang Z, et al.Prevalence of orofacial clefts among live births in China: a systematic review and meta-analysis[J]. Birth Defects Res, 2017, 109(13): 1011-1019.
[38] Gajera M, Desai N, Suzuki A, et al.MicroRNA-655-3p and microRNA-497-5p inhibit cell proliferation in cultured human lip cells through the regulation of genes related to human cleft lip[J]. BMC Med Genomics, 2019, 12(1): 70-87.
[39] Fu C, Lou S, Zhu G, et al.Identification of new miRNA-mRNA networks in the development of non-syndromic cleft lip with or without cleft palate[J]. Front Cell Dev Biol, 2021, 9: 631057.
[40] 王俊伟, 杨学财, 李玮, 等. 非综合征性唇腭裂外周血血浆外泌体源miRNA的表达研究[J]. 现代口腔医学杂志, 2019, 33(5): 261-265.

外泌体miRNA在口腔疾病中的研究进展

Research progress of exosomal miRNA in oral diseases

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