中国口腔颌面外科杂志

中国口腔颌面外科杂志 ›› 2022, Vol. 20 ›› Issue (2): 105-110.doi: 10.19438/j.cjoms.2022.02.001

• 论著 • 上一篇    下一篇

人牙髓干细胞来源外泌体促进周围神经损伤修复的实验研究

柴盈, 刘芷扬, 刘玥旻, 陈敏洁, 杨驰   

  1. 上海交通大学医学院附属第九人民医院 口腔外科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海 200011
  • 收稿日期:2021-08-09 修回日期:2021-11-10 出版日期:2022-03-20 发布日期:2022-03-20
  • 通讯作者: 陈敏洁,E-mail：chenmj_sub@163.com
  • 作者简介:柴盈（1988-）,女,硕士,主治医师,E-mail:chaiying_cherry@163.com
  • 基金资助:
    国家自然科学基金（81870785）; 上海市科委生物医药重点项目（16411953300）; 上海交通大学医学院附属第九人民医院临床研究型MDT项目（201701018）

Effect of exosomes derived from human dental pulp stem cells on peripheral nerve regeneration after crush injury

CHAI Ying, LIU Zhi-yang, LIU Yue-min, CHEN Min-jie, YANG Chi   

  1. Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China
  • Received:2021-08-09 Revised:2021-11-10 Online:2022-03-20 Published:2022-03-20

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摘要： 目的:研究人牙髓干细胞（human dental pulp stem cells,hDPSCs）来源外泌体对周围神经损伤修复的作用。方法:将36只成年SD大鼠随机分为2组,实验组为坐骨神经损伤后hDPSCs来源外泌体注射组,对照组为坐骨神经损伤后PBS注射组。分别于损伤后第3、7、14天观察大鼠的行为学改变,取材后进行H-E染色和免疫组织化学染色,观察神经束变化情况,透射电镜观察髓鞘改变。数据采用SPSS 24.0软件包进行单因素方差分析。结果:行为学观察发现,2组大鼠坐骨神经损伤后坐骨神经功能指数（sciatic functional index,SFI）下降,随时间延长,损伤后2周2组均基本恢复,但实验组较对照组恢复更快（P<0.05）。H-E染色和免疫组织化学染色提示,损伤后2组均出现神经束形态破坏、束膜不规则,但实验组神经束破坏程度较对照组轻,且恢复速度更快。电镜观察提示,损伤后2组均出现髓鞘崩解、水肿、变性,但实验组髓鞘的恢复速度较对照组加快。结论:hDPSCs来源外泌体能够促进周围神经损伤后修复,其机制可能与加快髓鞘再生有关。

关键词: 牙髓干细胞, 外泌体, 周围神经损伤, 髓鞘

Abstract: PURPOSE: To study the effect of exosomes derived from human dental pulp stem cells (hDPSCs) on recovery of peripheral nerve crush injury. METHODS: Thirty-six adult SD rats were randomly divided into two groups. Group A was treated with exosomes derived from hDPSCs after sciatic nerve injury, while group B was treated with PBS after sciatic nerve injury. The behavioral changes of rats were observed at 3, 7 and 14 d after injury. The nerve tracts were observed by H-E staining and immunohistochemical staining, and the changes of myelin sheath were observed by electron microscope. The data were analyzed by one-way ANOVA with SPSS 24.0 software package. RESULTS: Behavioral observation showed that the sciatic functional index(SFI) decreased in both groups after injury. With the time going on, the two groups basically recovered 2 weeks after injury, but the recovery in group A was faster than group B(P<0.05). H-E staining and immunohistochemical staining showed that after injury, nerve tract morphological damage and irregular perineurium appeared in both groups, but the degree of perineurium damage in group A was lighter than group B, and the recovery speed was faster. Electron microscopic observation showed that myelin disintegration, edema and degeneration occurred in both groups after injury, but the recovery speed of myelin in group A was faster than group B. CONCLUSIONS: Exosomes derived from hDPSCs can promote recovery of peripheral nerve injury, and its mechanism may be related to accelerating the regeneration of myelin sheath.

Key words: Dental pulp stem cells, Exosomes, Peripheral nerve injury, Myelin sheath

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