外源性β-NGF对骨缺损区骨形成和改建的调节作用

doi:10.19438/j.cjoms.2017.03.001

中国口腔颌面外科杂志 ›› 2017, Vol. 15 ›› Issue (3): 193-197.doi: 10.19438/j.cjoms.2017.03.001

外源性β-NGF对骨缺损区骨形成和改建的调节作用

卢萌*，卓丽莉*，茅传青，王锦，王承勇，陈伟辉

福建医科大学附属协和医院口腔颌面外科，福建福州 350001

收稿日期:2016-07-25 修回日期:2016-11-08 出版日期:2017-05-20 发布日期:2017-06-09
通讯作者: 国家自然科学基金（31070838）；福建省自然基金科学杰出青年项目（2012J06017）；福建省卫生厅青年基金（2010-1-10）
作者简介:卢萌（1983-），女，硕士，E-mail：17817017@qq.com；卓丽莉（1987-），女，硕士，E-mail：491799702@qq.com。*并列第一作者
基金资助:
陈伟辉，E-mail：dr_whchen@yahoo.com

Effect of β-NGF on bone formation and bone remodeling during healing of bone defect

LU Meng, ZHUO Li-li, MAO Chuan-qing, WANG Jin, WANG Cheng-yong, CHEN Wei-hui.

Department of Oral and Maxillofacial Surgery, Union Hospital affiliated to Fujian Medical University. Fuzhou 250001, Fujian Province, China

Received:2016-07-25 Revised:2016-11-08 Online:2017-05-20 Published:2017-06-09

摘要/Abstract

摘要： 目的：探讨β-NGF对骨缺损区新骨形成和改建的影响。方法：利用手术方法建立大鼠颅骨骨缺损模型，采用微渗透泵于骨缺损区局部持续导入外源性β-NGF。通过H-E染色、Gomori改良三色染色技术，检测术后3、7、14、21和28 d不同时间点骨缺损区的新生骨量，探讨β-NGF对骨缺损区新骨形成和改建的影响。应用Image-Pro Plus 6.0软件对骨缺损区中兴趣区域的新骨形成进行半定量测定，采用SPSS 19.0软件包对所得数据进行统计分析。结果：成功建立大鼠颅骨骨缺损局部持续导入β-NGF给药模型。H-E染色结果显示，导入β-NGF侧（实验组）与无β-NGF导入侧（对照组）均有新骨形成。Gomori改良三色染色结果发现，21、28 d时，实验组与对照组的新骨形成呈现明显增加，且实验组的新骨形成量显著多于对照组（P<0.05）， 28 d时的成熟骨量实验组显著多于对照组（P＜0.05）。结论：骨缺损区导入外源性β-NGF可能会促进新骨形成与骨改建。

关键词: β-NGF, 骨缺损, 骨形成, 骨改建

Abstract: PURPOSE: Standard rat skull critical bone defects were made and locally continuous infusion of β-NGF animal model was conducted. The effect of β-NGF on bone formation and bone remodeling was evaluated. METHODS: Two 5 mm diameter bone defects were created on the parietal bone of SD rats, the right side was given 10 μg β-NGF PBS solution(experimental group) and the left side was only given PBS solution(control group) via osmotic pump for 7 days. New bone formation was evaluated using H-E staining and Gomori modified trichrome staining. New bone formation in the bone defect of AOI was measured with Image-pro Plus 6.0 software and the data were analyzed using SPSS 19.0 software package. RESULTS: H-E staining showed the new bone formation on the both sides. The results of Gomori modified trichrome staining showed significantly increased new bone formation after application of β-NGF at 21 d and 28 d. The quality of mature bone was significantly higher in the experiment group compared with the control group at 28 d(P<0.05). CONCLUSIONS: Local application of β-NGF during bone healing can promote new bone formation and bone remodeling.

Key words: β-NGF, Bone defects, Bone formation, Bone remodeling

中图分类号:

R782

卢萌*，卓丽莉*，茅传青，王锦，王承勇，陈伟辉. 外源性β-NGF对骨缺损区骨形成和改建的调节作用[J]. 中国口腔颌面外科杂志, 2017, 15(3): 193-197.

LU Meng, ZHUO Li-li, MAO Chuan-qing, WANG Jin, WANG Cheng-yong, CHEN Wei-hui.. Effect of β-NGF on bone formation and bone remodeling during healing of bone defect[J]. China J Oral Maxillofac Surg, 2017, 15(3): 193-197.

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外源性β-NGF对骨缺损区骨形成和改建的调节作用

Effect of β-NGF on bone formation and bone remodeling during healing of bone defect

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