大豆异黄酮对颌骨骨髓源巨噬细胞破骨细胞向分化的影响

doi:10.19438/j.cjoms.2021.03.002

中国口腔颌面外科杂志 ›› 2021, Vol. 19 ›› Issue (3): 197-200.doi: 10.19438/j.cjoms.2021.03.002

大豆异黄酮对颌骨骨髓源巨噬细胞破骨细胞向分化的影响

陈富民, 代庆刚, 邬春兰^*, 王跃平^*

上海交通大学医学院附属第九人民医院口腔第二门诊部,上海交通大学口腔医学院, 国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海 200011

收稿日期:2021-01-22 修回日期:2021-03-22 发布日期:2021-07-16
通讯作者: 王跃平,E-mail：wangyueping309@hotmail.com;邬春兰, E-mail： wuchunlan9229@163.com。^*共同通信作者
作者简介:陈富民（1972-）,男,硕士,主治医师,E-mail：195812872@qq.com
基金资助:
国家自然科学基金（81800949）

Effect of soybean isoflavoneon on osteoclastic differentiation of mandible bone marrow-derived macrophages

CHEN Fu-min, DAI Qing-gang, WU Chun-lan, WANG Yue-ping

Second Dental Center, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2021-01-22 Revised:2021-03-22 Published:2021-07-16

摘要/Abstract

摘要： 目的评价大豆异黄酮对大鼠下颌骨骨髓源巨噬细胞（mBMMs）破骨细胞向分化的影响。方法采用CCK-8法检测mBMMs在100 μmol/L、10 μmol/L、1 μmol/L、100 nmol/L、10 nmol/L及1 nmol/L 大豆异黄酮作用下细胞活性的改变。通过RANKL刺激mBMMs破骨细胞向分化,利用TRAP染色等方法分析不同浓度大豆异黄酮作用下多核破骨细胞数目及破骨相关基因表达的改变。采用SPSS 16.0软件包对数据进行统计学分析。结果 1 μmol/L、100 nmol/L、 10 nmol/L及1 nmol/L大豆异黄酮组mBMMs细胞活性与对照组比较,无显著差异;而高浓度大豆异黄酮（100 μmol/L及10 μmol/L）可抑制mBMMs细胞活性（P<0.05）。mBMMs 在RANKL的诱导下形成破骨细胞,TRAP染色显示为酒红色多核巨细胞。与对照组相比,1 μmol/L及100 nmol/L大豆异黄酮组破骨细胞数目显著减少（P<0.05）。分析破骨相关基因表达,发现1 μmol/L及100 nmol/L大豆异黄酮均能降低NFATc1、Mmp9、Trap及Ctsk mRNA的表达水平（P<0.05）。结论大豆异黄酮可抑制mBMMs破骨细胞向分化。

关键词: 大豆异黄酮, 颌骨骨髓源巨噬细胞, 破骨细胞向分化, 颌骨骨质疏松症

Abstract: PURPOSE: To study the effects of soybean isoflavoneon on osteoclastic differentiation of mandible bone marrow-derived macrophages (mBMMs). METHODS: Four-week-old female S-D rats were used to obtain mBMMs. CCK-8 assay was used to analyze the changes of cell viability of mBMMs due to different concentration of soybean isoflavoneon. The effect of soybean isoflavoneon on osteoclastic formation and osteoclastic gene expression was determined by tartrate resistant acid phosphatase staining (TRAP) and q-PCR. SPSS16.0 software package was used for data analysis. RESULTS: Cell viability of mBMMs treated with 1 μmol/L, 100 nmol/L, 10 nmol/L and 1 nmol/L soybean isoflavoneon was comparable with the control group, while100 μmol/L and10 μmol/L soybean isoflavoneon inhibited cell viability of mBMMs (P<0.05). With TRAP staining, both 1 μmol/L and 100 nmol/L soybean isoflavoneon decreased the number osteoclast in BMMs induced by RANKL (P<0.05). Moreover, the mRNA level of NFATc1, MMP9, Trap and Ctsk of in mBMMs treated with 1 μmol/L and 100 nmol/L soybean isoflavoneon decreased significantly in comparison with the control group. CONCLUSIONS: Soybean isoflavoneon inhibits osteoclastic differentiation of mBMMs.

Key words: Soybean isoflavoneon, Mandible bone marrow-derived macrophages, mBMMs, Osteoclastic differentiation, Osteoporosis

中图分类号:

R782.3.2

陈富民, 代庆刚, 邬春兰, 王跃平. 大豆异黄酮对颌骨骨髓源巨噬细胞破骨细胞向分化的影响[J]. 中国口腔颌面外科杂志, 2021, 19(3): 197-200.

CHEN Fu-min, DAI Qing-gang, WU Chun-lan, WANG Yue-ping. Effect of soybean isoflavoneon on osteoclastic differentiation of mandible bone marrow-derived macrophages[J]. China J Oral Maxillofac Surg, 2021, 19(3): 197-200.

参考文献

[1] Dai QG, Zhang P, Wu YQ, et al.Ovariectomy induces osteoporosis in the maxillary alveolar bone: an in vivo micro-CT and histomorphometric analysis in rats[J]. Oral Dis, 2014, 20(5): 514-520.
[2] Zhou S, Yang Y, Ha N, et al.The specific morphological features of alveolar bone[J]. J Craniofac Surg, 2018, 29(5): 1216-1219.
[3] Santana N, Mehazabin S, Sangeetha K, et al.Osteodystrophies of jaws[J]. J Oral Maxillofac Pathol, 2020, 24(2): 405-412.
[4] Xu H, Zhou S, Qu R, et al.Icariin prevents oestrogen deficiency-induced alveolar bone loss through promoting osteogenesis via STAT3[J]. Cell Prolif, 2020, 53(2): e12743.
[5] Stewart S, Hanning R.Building osteoporosis prevention into dental practice[J]. J Can Dent Assoc, 2012, 78: c29-c40.
[6] Guiglia R, Di Fede O, Lo Russo L, et al.Osteoporosis, jawbones and periodontal disease[J]. Med Oral Patol Oral Cir Bucal, 2013, 18(1): e93-e99.
[7] Koduganti RR, Gorthi C, Reddy PV, et al.Osteoporosis: "A risk factor for periodontitis"[J]. J Indian Soc Periodontol, 2009, 13(2): 90-96.
[8] Rachner TD, Khosla S, Hofbauer LC.Osteoporosis: now and the future[J]. Lancet, 2011, 377(9773): 1276-1287.
[9] NIH Consensus Development Panel on Osteoporosis Prevention, Diagnosis,Therapy. Osteoporosis prevention, diagnosis, and therapy[J]. JAMA, 2001, 285(6): 785-795.
[10] Brown JP, Josse RG.2002 clinical practice guidelines for the diagnosis and management of osteoporosis in Canada[J]. CMAJ, 2002, 167(10 Suppl): S1-S34.
[11] Zheng X, Lee SK, Chun OK.Soy isoflavones and osteoporotic bone loss: a review with an emphasis on modulation of bone remodeling[J]. J Med Food, 2016, 19(1): 1-14.
[12] Li HF, Zhang P, Tian ZF, et al.Differential mechanisms involved in effects of genistein and 17-beta-estradiol on porcine coronary arteries[J]. Pharmazie, 2006, 61(5): 461-465.
[13] Yamaguchi M, Weitzmann MN.The estrogen 17beta-estradiol and phytoestrogen genistein mediate differential effects on osteoblastic NF-kappaB activity[J]. Int J Mol Med, 2009, 23(2): 297-301.
[14] Liu K, Ma G, Lv G, et al.Effects of soybean isoflavone dosage and exercise on the serum markers of bone metabolism in ovariectomized rats[J]. Asia Pac J Clin Nutr, 2007, 16( Suppl 1): 193-195.
[15] Uesugi T, Fukui Y, Yamori Y.Beneficial effects of soybean isoflavone supplementation on bone metabolism and serum lipids in postmenopausal japanese women: a four-week study[J]. J Am Coll Nutr, 2002, 21(2): 97-102.
[16] 陶若奇, 张建东, 顾九君, 等, 大豆异黄酮对去势大鼠骨质疏松性骨折愈合的影响[J]. 临床骨科杂志, 2015, 18(1): 110-113.
[17] Sliwinski L, Folwarczna J, Janiec W, et al.Differential effects of genistein, estradiol and raloxifene on rat osteoclasts in vitro[J]. Pharmacol Rep, 2005, 57(3): 352-359.
[18] Katz J, Rotstein I.Prevalence of periapical lesions in patients with osteoporosis[J]. J Endod, 2021, 47(2): 234-238.
[19] Amaro ERS, Ortiz FR, Dorneles LS, et al. Estrogen protects dental roots from orthodontic-induced inflammatory resorption[J]. Arch Oral Biol, 2020, 117: 104820.e1-e8.
[20] Durie BGM, Katz M, Crowley J, et al.Osteonecrosis of the jaw and bisphosphonates[J]. N Engl J Med, 2005, 353(1): 99-102.
[21] Chien HI, Chen LW, Liu WC, et al.Bisphosphonate-related osteonecrosis of the jaw[J]. Ann Plast Surg, 2021, 86(2S Suppl 1): S78-S83.
[22] Ryu JI, Kim HY, Kwon YD.Is implant surgery a risk factor for osteonecrosis of the jaw in older adult patients with osteoporosis? A national cohort propensity score-matched study[J]. Clin Oral Implants Res, 2021, 32(4): 437-447.
[23] Yu F, Liu Z, Tong Z, et al.Soybean isoflavone treatment induces osteoblast differentiation and proliferation by regulating analysis of Wnt/beta-catenin pathway[J]. Gene, 2015, 573(2): 273-277.
[24] Lu M, Xie K, Huang K, et al.Effects of soybean isoflavone on metabolism of rat osteoblasts and cytokines in vitro[J]. J Food Sci, 2020, 85(4): 1302-1306.

大豆异黄酮对颌骨骨髓源巨噬细胞破骨细胞向分化的影响

Effect of soybean isoflavoneon on osteoclastic differentiation of mandible bone marrow-derived macrophages

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价