应用齿贝骨粉及矿化胶原膜修复小型猪拔牙创骨缺损的效果观察

doi:10.19438/j.cjoms.2017.05.006

中国口腔颌面外科杂志 ›› 2017, Vol. 15 ›› Issue (5): 408-412.doi: 10.19438/j.cjoms.2017.05.006

应用齿贝骨粉及矿化胶原膜修复小型猪拔牙创骨缺损的效果观察

王飘¹，姚瑶²，赵皓³，潘巨利⁴，崔福斋⁵，郭亚娟⁶

1.首都医科大学附属北京口腔医院整形创伤外科，2.修复科北京 100050;
3.广东省口腔医院·南方医科大学附属口腔医院，广东广州 510280;
4.首都医科大学附属北京口腔医院多学科诊疗中心，北京 100050;
5.清华大学材料科学与工程系，北京 100084;
6.国家食品药品监督管理总局医疗器械技术审评中心，北京 100081

收稿日期:2016-12-08 出版日期:2017-08-30 发布日期:2017-10-27
通讯作者: 郭亚娟，E-mail:guoyj@cmde.org.cn
作者简介:王飘（1985-），女，硕士，主治医师，E-mail：wangpiao850724@163.com

Repair of bone defect by using mineralized collagen materials and collagen membrane in minipig models

WANG Piao¹, YAO Yao², ZHAO Hao³, PAN Ju-li⁴, CUI Fu-zhai⁵，GUO Ya-juan⁶.

1.Department of Oral and Maxillofacial Plastic & Trauma Surgery, 2.Department of Prosthodontics, School of Stomatology, Capital Medical University. Beijing 100050;
3.Guangdong Provincial Stomatological Hospital, Southern Medical University. Guangzhou 510280, Guangdong Province;
4.Multidisciplinary Treatment Center, Capital Medical University. Beijing 100050;
5.School of Materials Science and Engineering, Tsinghua University. Beijing 100084;
6. Center For Medical Device Evaluation, CFDA. Beijing 100081,China

Received:2016-12-08 Online:2017-08-30 Published:2017-10-27

摘要/Abstract

摘要： 目的：比较 Bio-Oss骨粉和齿贝骨粉修复拔牙窝骨缺损的成骨效果，评估齿贝牙科骨粉在拔牙窝骨缺损修复中的可靠性。方法：以引导骨再生术（GBR）为基本原理，在小型猪拔牙区制造骨缺损，分别植入齿贝牙科骨粉及Bio-Oss骨粉。术后12周进行肉眼观察、影像学检查及组织学染色。影像学检查测量骨缺损的成骨密度，采用SPSS 22.0软件包进行配对t检验，观察骨缺损修复情况。分析组织切片中新生骨在骨缺损区域所占面积的百分比，得出新生骨百分率，比较2种不同材料骨粉修复拔牙窝骨缺损的效果。结果：术后12周，CT显示不同组的拔牙窝骨缺损完全被新生骨填充，2种骨粉修复骨缺损效果无显著差异。组织学切片显示，与Bio-Oss骨粉相比，齿贝骨粉填充骨缺损区成骨量略有减少，但两者差异不显著。结论：齿贝牙科骨粉在拔牙窝骨缺损修复中具有可靠性，有必要做进一步临床研究。

关键词: 拔牙创位点保存, 骨缺损修复, 齿贝骨粉, 引导骨组织再生

Abstract: PURPOSE: The aim of this study was to compare the clinical and histologic outcomes of bone regeneration using two different graft materials: deproteinized bovine bone mineral (Bio-Oss) and mineralized collagen bone materials (ChiBei) with absorbable collagen membrane, thus to evaluate the effect of ChiBei on bone regeneration. METHODS: The molars of the minipig were extracted, a 10mm×10mm bone defect was created. Bone defect was filled with Bio-Oss and ChiBei respectively and with absorbable collagen membrane as well. Cone-beam CT (CBCT) was taken 12 weeks after surgery and bone samples were stained with Goldner's Trichrome. The new regenerated area was calculated by the image analysis software Image-Pro plus 6.0 and the percentages of newly formed bone were compared between two groups using SPSS 22.0 software package. RESULTS: Bone defect was filled with new regenerated bone in each group. There was no significant difference in bone density between the ChiBei and Bio-Oss group on CBCT, so did the histologic analysis results. CONCLUSIONS: Based on the findings of the study, we speculated ChiBei materials covered with collagen membrane have positive effect on bone regeneration after tooth extraction, but more researches need to be carried out.

Key words: Site preservation, Bone defect regeneration, ChiBei materials, Guided bone regeneration

中图分类号:

R782.1

王飘，姚瑶，赵皓，潘巨利，崔福斋，郭亚娟. 应用齿贝骨粉及矿化胶原膜修复小型猪拔牙创骨缺损的效果观察[J]. 中国口腔颌面外科杂志, 2017, 15(5): 408-412.

WANG Piao, YAO Yao, ZHAO Hao, PAN Ju-li, CUI Fu-zhai，GUO Ya-juan.. Repair of bone defect by using mineralized collagen materials and collagen membrane in minipig models[J]. China J Oral Maxillofac Surg, 2017, 15(5): 408-412.

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应用齿贝骨粉及矿化胶原膜修复小型猪拔牙创骨缺损的效果观察

Repair of bone defect by using mineralized collagen materials and collagen membrane in minipig models

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