中国口腔颌面外科杂志

中国口腔颌面外科杂志 ›› 2017, Vol. 15 ›› Issue (5): 413-415.doi: 10.19438/j.cjoms.2017.05.007

• 论著 • 上一篇    下一篇

Maspin基因甲基化对口腔黏膜鳞癌细胞的调控作用

罗军，吴伟力，应于康， 徐旭辉，朱海钱   

  1. 浙江省台州市中心医院 口腔科，浙江 台州 318000
  • 收稿日期:2016-11-28 出版日期:2017-08-30 发布日期:2017-10-27
  • 通讯作者: 罗军，E-mail:noaste@163.com
  • 作者简介:罗军（1977-），男，硕士，副主任医师

Involvement of Maspin methylation in the proliferation of oral squamous cell carcinoma

LUO Jun, WU Wei-li, YＩＮＧ Yu-kang, XU Xu-hui, ZHU Hai-qian.   

  1. Department of Stomatology, Taizhou Central Hospital. Taizhou 318000, Zhejiang Province, China
  • Received:2016-11-28 Online:2017-08-30 Published:2017-10-27

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摘要： 目的：分析口腔黏膜鳞癌细胞增殖过程中Maspin基因甲基化的调控作用机制，为相关研究及临床治疗提供借鉴。方法：鳞状细胞癌HIOEC-B(a)P细胞系随机分为4组：5-氮-2-脱氧胞苷+曲古抑菌素A(ADC+TSA)阴性组、低(0.1 μmol/L +0.05 μmol/L)、中(1 μmol/L+0.5 μmol/L)和高剂量组(10 μmol/L+5 μmol/L)。以正常口腔黏膜上皮细胞作为对照组。实时定量PCR检测各组细胞Maspin基因的甲基化程度，MTT法检测各组细胞的增殖情况，4',6-二脒基-2-苯基吲哚（DAPI）染色检测细胞凋亡。采用SPSS20.0软件包对数据进行单因素方差分析。结果：与对照组相比，癌细胞的Maspin基因甲基化程度显著增强（P<0.01），与阴性组相比，中剂量和高剂量组Maspin基因甲基化程度显著减弱（P<0.05，P<0.01），低、中、高剂量组的细胞增殖抑制率显著高于阴性组（P<0.05，P<0.01）。随着药物剂量的提高，细胞凋亡程度逐渐增加（P<0.05）。结论：Maspin基因甲基化可能参与口腔黏膜鳞癌细胞的增殖过程。

关键词: Maspin基因, 甲基化, 5-氮-2-脱氧胞苷, 曲古抑菌素A

Abstract: PURPOSE: Explore the involvement of Maspin methylation in the proliferation of oral squamous cell carcinoma. METHODS: HIOEC-B(a)P cell line was divided into 4 groups: negative control, ADC+TSA with low (0.1 μmol/L+0.05 μmol/L), middle (1 μmol/L+0.5 μmol/L) and high (10 μmol/L+5 μmol/L) dose. Oral epithelial cells were taken as control. Methylation degree was detected by real-time PCR. The proliferation and apoptosis were detected by MTT and DAPI staining. The data were analyzed using SPSS 20.0 software package. RESULTS: Maspin methylation was enhanced in tumor cells when compared with control group (P<0.01). Maspin methylation was decreased in middle and high dose group when compared with negative group (P<0.05, P<0.01). The inhibition ratio of cell multiplication was enhanced when compared with negative group (P<0.05, P<0.01). The apoptosis content was enhanced after incubation with ADC+TSA with a dose dependent manner (P<0.05). CONCLUSIONS: Methylation of Maspin gene may be involved in the proliferation of oral cancer cells.

Key words: Maspin gene, Methylation, ADC, TSA

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