核糖体蛋白L29在舌鳞状细胞癌中的表达及意义

doi:10.19438/j.cjoms.2021.02.003

中国口腔颌面外科杂志 ›› 2021, Vol. 19 ›› Issue (2): 110-115.doi: 10.19438/j.cjoms.2021.02.003

核糖体蛋白L29在舌鳞状细胞癌中的表达及意义

孔宪琛^1,2, 姜晓东³, 芦昕², 禹雯怡^1,2, 薛娇², 张鹏², 赵璐², 袁荣涛²

1.青岛大学口腔医学院,山东青岛 266003;
2.青岛大学附属青岛市市立医院口腔医学中心,山东青岛 266071;
3.青岛市第五人民医院口腔科,山东青岛 266002

收稿日期:2020-09-21 修回日期:2020-11-16 出版日期:2021-03-20 发布日期:2021-05-11
通讯作者: 袁荣涛,E-mail：yuanrongtao@163.com
作者简介:孔宪琛（1993-）,男,硕士,医师,E-mail：kxcmtin@163.com
基金资助:
山东省自然科学基金（ZR2016HM34）

Expression and significance of ribosomal protein L29 in tongue squamous cell carcinoma

KONG Xian-chen^1,2, JIANG Xiao-dong³, LU Xin², YU Wen-yi², XUE Jiao², ZHANG Peng², ZHAO Lu², YUAN Rong-tao²

1. School of Stomatology, Qingdao University. Qingdao 266003;
2. Stomatology Center, Qingdao Municipal Hospital. Qingdao 266071;
3. Department of Stomatology, Qingdao Fifth People’s Hospital. Qingdao 266002, Shandong Province, China

Received:2020-09-21 Revised:2020-11-16 Online:2021-03-20 Published:2021-05-11

摘要/Abstract

摘要： 目的探讨核糖体蛋白L29(ribosomal protein L29,RPL29)在人舌鳞状细胞癌（简称鳞癌）中的表达及意义。方法应用免疫组织化学SP法检测52例舌鳞癌患者癌组织和癌旁组织中RPL29蛋白的表达情况。利用RNA干扰技术,将RPL29-siRNA转染到舌鳞癌CAL-27细胞中,RT-PCR法检测RPL29 mRNA的表达,蛋白质印迹法检测RPL29蛋白的表达,Transwell小室检测细胞的侵袭能力。采用 SPSS 21.0 软件包对数据进行统计学处理。结果 RPL29在舌鳞癌组织中的表达显著高于癌旁组织,RPL29蛋白表达与肿瘤有、无淋巴结转移相关（P<0.05）,与患者性别、年龄、吸烟史、饮酒史、病理分级、T分期无显著相关性（P>0.05）。转染RPL29-siRNA后,舌鳞癌CAL-27细胞中RPL29表达水平下调, CAL-27细胞的侵袭能力显著下降。结论 RPL29在舌鳞癌组织中表达增高,其表达水平与患者有、无淋巴结转移之间差异显著。抑制舌鳞癌CAL-27细胞中RPL29的表达,可降低细胞体外侵袭能力。RPL29在舌鳞癌的侵袭和转移中可能发挥重要作用。

关键词: 核糖体蛋白 L29, 舌鳞状细胞癌, RNA干扰, 侵袭

Abstract: PURPOSE: To investigate the expression and significance of ribosomal protein L29 (RPL29) in human tongue squamous cell carcinoma (TSCC). METHODS: SP method of immunohistochemistry was carried out to detect the expression of RPL29 in cancer tissues and adjacent tissues of 52 TSCC patients. CAL-27 cells were transfected with RPL29-siRNA. RPL29 protein and mRNA levels were detected using Western blot and RT-PCR. Transwell invasion assay was performed to assess the invasion ability of CAL-27 cells. SPSS 21.0 software package was used for statistical analysis. RESULTS: The expression of RPL29 in TSCC was significantly higher than that in paracancerous tissues. RPL29 over-expression was found to be associated with cervical lymph node metastasis（P<0.05）,but not with age,gender,smoking history,alcohol drinking history, pathological type and T stage(P>0.05). After transfecting RPL29-siRNA into CAL-27 cells, the ability of invasion was significantly decreased. CONCLUSIONS: The expression of RPL29 in TSCC was significantly increased and its expression level was significantly different from that in patients with or without lymph node metastasis. The invasive ability of CAL-27 cells was significantly inhibited after transfection with RPL29-siRNA. RPL29 may play an important role in the processes of invasion and metastasis of TSCC.

Key words: Ribosomal protein L29, Tongue squamous cell carcinoma, RNA interference, Invasion

中图分类号:

R739.8

孔宪琛, 姜晓东, 芦昕, 禹雯怡, 薛娇, 张鹏, 赵璐, 袁荣涛. 核糖体蛋白L29在舌鳞状细胞癌中的表达及意义[J]. 中国口腔颌面外科杂志, 2021, 19(2): 110-115.

KONG Xian-chen, JIANG Xiao-dong, LU Xin, YU Wen-yi, XUE Jiao, ZHANG Peng, ZHAO Lu, YUAN Rong-tao. Expression and significance of ribosomal protein L29 in tongue squamous cell carcinoma[J]. China J Oral Maxillofac Surg, 2021, 19(2): 110-115.

参考文献

[1] 郑家伟, 李金忠, 钟来平, 等. 口腔鳞状细胞癌临床流行病学研究现状[J]. 中国口腔颌面外科杂志, 2007, 5(2): 83-90.
[2] Spiotto MT, Jefferson G, Wenig B, et al.Differences in survival with surgery and postoperative radiotherapy compared with definitive chemoradiotherapy for oral cavity cancer: a national cancer database analysis[J]. JAMA Otolaryngol Head Neck Surg, 2017, 143(7): 691-699.
[3] Warnakulasuriya S.Global epidemiology of oral and oropharyngeal cancer[J]. Oral Oncol, 2009, 45(4-5): 309-316.
[4] Liu JJ, Zhang J, Ramanan S, et al.Heparin/heparan sulfate interacting protein plays a role in apoptosis induced by anticancer drugs[J]. Carcinogenesis, 2004, 25(6): 873-879.
[5] 尚伟, 郑家伟. 口腔及口咽癌新版TNM分期与NCCN诊治指南部分解读[J]. 中国口腔颌面外科杂志, 2018, 16(6): 533-546.
[6] Liu LK, Jiang XY, Zhou XX, et al.Upregulation of vimentin and aberrant expression of E-cadherin/b-catenin complex in oral squamous cell carcinomas: correlation with the clinicopathological features and patient outcome[J]. Mod Pathol, 2010, 23(2): 213-224.
[7] Schmittgen TD, Livak KJ.Analyzing real-time PCR data by the comparative C(T) method[J]. Nat Protoc, 2008, 3(6): 1101-1108.
[8] Ng JH, Iyer NG, Tan MH, et al.Changing epidemiology of oral squamous cell carcinoma of the tongue: a global study[J]. Head Neck, 2016, 39(2): 297-304.
[9] Rusthoven K, Ballonoff A, Raben D, et al.Poor prognosis in patients with stage Ⅰ and Ⅱ oral tongue squamous cell carcinoma[J]. Cancer, 2008, 112(2): 345-351.
[10] 李江. 口腔癌的临床病理特征与预后[J]. 中国口腔颌面外科杂志, 2008, 6(1): 17-21.
[11] Kasai H, Nadano D, Hidaka E, et al.Differential expression of ribosomal proteins in human normal and neoplastic colorectum[J]. J Histochem Cytochem, 2003, 51(5): 567-574.
[12] Kobayashi T, Sasaki Y, Oshima Y, et al.Activation of the ribosomal protein L13 gene in human gastrointestinal cancer[J]. Int J Mol Med, 2006, 18(1): 161-170.
[13] Delabre ML, Kessl J, Karamanou S, et al.RPL29 codes for a non-essential protein of the 60S ribosomal subunit in Saccharomyces cerevisiae and exhibits synthetic lethality with mutations in genes for proteins required for subunit coupling[J]. Biochim Biophys Acta, 2002,1574(3): 255-261.
[14] Wang Y, Cheong D, Chan S, et al.Heparin/heparan sulfate interacting protein gene expression is up- regulated in human colorectal carcinoma and correlated with differentiation status and metastasis[J]. Cancer Res, 1999, 59(12): 2989-2994.
[15] Nigris FD, Visconti R, Cerutti J, et al.Overexpression of the HIP gene coding for a heparin/heparan sulfate-binding protein in human thyroid carcinomas[J]. Cancer Res, 1998, 58(20): 4745-4751.
[16] Zeng H, Sanyal S, Mukhopadhyay D.Tyrosine residues 951 and 1059 of vascular endothelial growth factor receptor-2 (KDR) are essential for vascular permeability factor/vascular endothelial growth factor-induced endothelium migration and proliferation, respectively[J]. J Biol Chem, 2001, 276(35): 32714-32719.
[17] D'Souza S, Yang W, Marchetti D, et al. HIP/RPL29 antagonizes VEGF and FGF2 stimulated angiogenesis by interfering with HS-dependent responses[J]. J Cell Biochem, 2008, 105(5): 1183-1193.
[18] 薛娇, 段青云, 杨绍滨, 等. 核糖体蛋白L29在牙龈鳞状细胞癌中表达的临床病理意义[J]. 口腔医学, 2018, 38(6): 508-513.
[19] Li CD, Ge M, Yin Y, et al.Silencing expression of ribosomal protein L26 and L29 by RNA interfering inhibits proliferation of human pancreatic cancer PANC-1 cells[J]. Mol Cell Biochem, 2012, 370(1-2): 127-139.

核糖体蛋白L29在舌鳞状细胞癌中的表达及意义

Expression and significance of ribosomal protein L29 in tongue squamous cell carcinoma

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