雷洛昔芬对牙周炎合并系统性绝经后骨质疏松症小鼠模型局部牙槽骨破坏的影响

doi:10.19438/j.cjoms.2020.05.005

中国口腔颌面外科杂志 ›› 2020, Vol. 18 ›› Issue (5): 407-411.doi: 10.19438/j.cjoms.2020.05.005

雷洛昔芬对牙周炎合并系统性绝经后骨质疏松症小鼠模型局部牙槽骨破坏的影响

杨正祥¹, 李航², 李鲲¹

1.成都市龙泉驿区第一人民医院（四川大学华西医院龙泉医院）口腔科,四川成都 610100;
2.成都市龙泉驿区妇幼保健院儿童保健科,四川成都 610100

收稿日期:2020-03-19 修回日期:2020-05-25 出版日期:2020-09-20 发布日期:2020-10-28
通讯作者: 杨正祥,E-mail:：93434002@qq.com
作者简介:杨正祥（1982-）,男,学士,主治医师

Effect of raloxifene on local alveolar bone destruction of mice with periodontitis and systemic postmenopausal osteoporosis

YANG Zheng-xiang¹, LI Hang², LI Kun¹

1. Department of Stomatology, the First People's Hospital of Longquanyi District of Chengdu City; West China Longquan Hospital Sichuan University. Chengdu 610100;
2. Department of Children Healthcare, Longquanyi District of Chengdu Maternity and Child Health Care Hospital. Chengdu 610100,Sichuan Province, China

Received:2020-03-19 Revised:2020-05-25 Online:2020-09-20 Published:2020-10-28

摘要/Abstract

摘要： 目的：探讨雷洛昔芬对牙周炎合并系统性绝经后骨质疏松症（PMO）小鼠模型局部牙槽骨破坏的影响。方法：无特定病原级雌性成熟Wistar小鼠,适应性喂养7 d 后,随机分为空白组（保留两侧卵巢,只切除卵巢组织周围与卵巢重量相当的脂肪组织）、对照组（切除两侧卵巢+口腔正畸结扎丝结扎）、实验组（切除两侧卵巢+口腔正畸结扎丝结扎+雷洛昔芬试剂）,每组各15只。术后4周拍摄CT,检测骨密度和骨吸收情况,体视显微镜观察牙槽骨破坏情况,酶联免疫吸附测定法（ELISA）检测破骨相关因子白细胞介素1(IL-1)、IL-1α、转化生长因子(TGF)、肿瘤坏死因子α(TNF-α)、TNF-β的表达水平。采用SPSS 18.0软件包对数据进行统计学分析。结果：对照组新骨形成少于实验组。亚甲蓝染色结果显示,对照组小鼠骨显著吸收,体视显微镜下可见骨破坏,实验组小鼠骨吸收程度较轻。与空白组相比,对照组、实验组牙槽骨密度降低而牙槽骨丧失、IL-1、IL-1α、TGF、TNF-α、TNF-β显著升高（P<0.05）;对照组牙槽骨密度显著低于实验组（P<0.05）,牙槽骨丧失、IL-1、IL-1α、TGF、TNF-α、TNF-β显著高于实验组（P<0.05）。结论：雷洛昔芬可减少PMO牙周炎症小鼠牙槽骨吸收、破骨细胞因子表达,防止牙槽骨破坏而有效防止牙周炎进展。

关键词: 雷洛昔芬, 牙周炎合并系统性绝经后骨质疏松症, 牙槽骨破坏

Abstract: PURPOSE: To investigate the effect of raloxifene on local alveolar bone destruction of mice with periodontitis and systemic postmenopausal osteoporosis (PMO). METHODS: No specific pathogen grade Wistar female rats purchased from Beijing Institute of Medical Device Testing were divided into 3 groups after 7 days of adaptive feeding, 15 cases in each group. Equal weight of peri-ovary fat tissue was cut in blank group; Rats in the control group received bilateral ovary resection combined with ligation with orthodontic ligation wire, while rats in experimental group received bilateral ovary resection + ligation with orthodontic ligation wire + raloxifene treatment. The activity, diet, weight and the incidence of tooth loosening were observed every day. Bone mineral density and bone resorption were measured by CT scanner, alveolar bone destruction was observed by stereomicroscope, and osteoclast related factors such as interleukin-1 (IL-1), IL-1α, transforming growth factor (TGF), tumor necrosis factor-α(TNF-α) and TNF-β were detected by enzyme-linked immunosorbent assay (ELISA). SPSS 18.0 software package was used for data analysis. RESULTS: New bone formation was the lowest in the control group, followed by experimental group and blank group. The results of methylene blue staining showed that bone resorption was significant in the control group, and bone destruction was also seen under stereomicroscope; bone resorption and bone destruction of the experimental group were better than those of the control group. Compared with the blank group, alveolar bone density decreased, alveolar bone loss, IL-1, IL-1α, TGF, TNF-α, TNF-β increased in control group and experimental group. The alveolar bone density of the control group was significantly lower than that of the experimental group (P<0.05), and alveolar bone loss, IL-1, IL-1α, TGF, TNF-α, and TNF-β were significantly higher than those of the experimental group (P<0.05). CONCLUSIONS: Raloxifene can reduce alveolar bone resorption and osteoclast cytokine expression in mice with PMO periodontal inflammation, prevent alveolar bone destruction and effectively prevent the progression of periodontitis.

Key words: Raloxifene, Periodontitis with systemic postmenopausal osteoporosis, Alveolar bone destruction

中图分类号:

R782.1

杨正祥, 李航, 李鲲. 雷洛昔芬对牙周炎合并系统性绝经后骨质疏松症小鼠模型局部牙槽骨破坏的影响[J]. 中国口腔颌面外科杂志, 2020, 18(5): 407-411.

YANG Zheng-xiang, LI Hang, LI Kun. Effect of raloxifene on local alveolar bone destruction of mice with periodontitis and systemic postmenopausal osteoporosis[J]. China J Oral Maxillofac Surg, 2020, 18(5): 407-411.

参考文献

[1] Leite FRM, Nascimento GG, Scheutz F, et al.Effect of smoking on periodontitis: A systematic review and meta-regression[J]. Am J Prev Med, 2018, 54(6): 831-841.
[2] Teixeira FV, Alves GL, Ferreira MH, et al.Preformulation studies to guide the development of raloxifene lipid-based delivery systems[J]. J Therm Anal Calorim, 2018, 132: 365-371.
[3] 骆凯, 闫福华, 陈凌, 等. 雷洛昔芬防治骨质疏松大鼠牙周炎牙槽骨吸收的实验研究[J]. 实用口腔医学杂志, 2008, 24(4): 491-495.
[4] Tatar O, Ilhan N, Ilhan N, et al.Is there any potential anticancer effect of raloxifene and fluoxetine on DMBAnduced rat breast cancer?[J]. J Biochem Mol Toxicol, 2019, 33(9): e22371.
[5] 杨霞, 向学熔, 李丛华, 等. 益气滋肾合剂对牙周炎大鼠外周血TNF-α、IL-β含量及牙槽骨微观形态的影响[J]. 第三军医大学学报, 2011, 33(13): 1378-1381.
[6] 白国辉, 杨智强, 曾凤娇, 等. 一种SD大鼠实验性牙周炎模型的建立方法[J]. 滨州医学院学报, 2019, 42(4): 241-242.
[7] Heikkilä P, But A, Sorsa T, et al.Periodontitis and cancer mortality: register-based cohort study of 68 273 adults in 10-year follow-up[J]. Int J Cancer, 2018, 142(11): 2244-2253.
[8] 吕雪雯, 潘春玲. 牙周组织工程的研究进展[J]. 中国医科大学学报, 2019, 48(12): 654-658.
[9] Posritong S, Hong JM, Eleniste PP, et al.Pyk2 deficiency potentiates osteoblast differentiation and mineralizing activity in response to estrogen or raloxifene[J]. Mole Cell Endocrinol, 2018, 474: 35-47.
[10] 郭峰, 李正南, 刘晋平, 等. 六月龄大鼠去卵巢后建立骨质疏松症模型的可行性[J]. 中国组织工程研究, 2012, 16(24): 4459-4462.
[11] 胡芳, 周淑, 龚爱秀. 实验性牙周炎对小鼠骨髓活性氧生成影响的实验研究[J]. 南京医科大学学报, 2019, 22(8):1147-1150.
[12] 颜明, 林妙阔. 实验性牙周炎大鼠模型的构建及脂氧素A4对牙槽骨吸收的影响[J]. 福建医药杂志, 2018, 40(3):142-144
[13] 蔡敏, 王翔宇, 苏浩, 等. 牙周基础治疗联合正畸对慢性牙周炎伴前牙病理性移位的治疗效果[J]. 中国实用医刊, 2019, 46(16): 69-71.
[14] Wu B, Shah SN, Lu Z, et al.Long-term treatment of tamoxifen and raloxifene alleviates dystrophic phenotype and enhances muscle functions of FKRP dystroglycanopathy[J]. Am J Pathol, 2018, 188(4): 1069-1080.
[15] 罗方. 雷洛昔芬联合碳酸钙D3治疗绝经后骨质疏松症的疗效研究[J]. 湖北科技学院学报(医学版), 2019, 33(1):36-38.
[16] 栾迪, 陈川进, 徐阳, 等. 选择性雌激素受体调节剂在脑缺血中的神经保护作用[J]. 国际脑血管病杂志, 2018, 26(5): 368-372.
[17] 张娟, 张志光, 李宏捷, 等. 小型猪一侧失牙及义齿修复后TMJ关节液中IL-1β、TNF-α、TGF-β1含量的变化[J]. 中华老年口腔医学杂志, 2008, 6(3): 167-171.

雷洛昔芬对牙周炎合并系统性绝经后骨质疏松症小鼠模型局部牙槽骨破坏的影响

Effect of raloxifene on local alveolar bone destruction of mice with periodontitis and systemic postmenopausal osteoporosis

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价