热化疗诱导舌鳞癌CAL-27细胞免疫原性死亡的实验研究

doi:10.19438/j.cjoms.2020.02.004

中国口腔颌面外科杂志 ›› 2020, Vol. 18 ›› Issue (2): 111-116.doi: 10.19438/j.cjoms.2020.02.004

热化疗诱导舌鳞癌CAL-27细胞免疫原性死亡的实验研究

孙巧珍^1,2, 石凡^1,2, 罗丹², 徐婷², 王升志²

1.青岛大学口腔医学院,山东青岛 266003;
2.青岛大学附属烟台毓璜顶医院口腔颌面外科,山东烟台 264000

收稿日期:2019-08-13 修回日期:2019-10-23 出版日期:2020-03-20 发布日期:2020-04-30
通讯作者: 王升志,E-mail：wangsz916@163.com
作者简介:孙巧珍（1992-）,女,在读硕士研究生,E-mail：283552476@qq.com
基金资助:
烟台市科技计划项目（2018YT06000220）

Study on immunogenic cell death induced by thermo-chemotherapy in CAL-27 cells of tongue squamous cell carcinoma

SUN Qiao-zhen^1,2, SHI Fan^1,2, LUO Dan², XU Ting², WANG Sheng-zhi²

1.School of Stomatology of Qingdao University. Qingdao 266003;
2.Department of Oral and Maxillofacial Surgery, Affiliated Yantai Yuhuangding Hospital of Qingdao University. Yantai 264000, Shandong Province, China

Received:2019-08-13 Revised:2019-10-23 Online:2020-03-20 Published:2020-04-30

摘要/Abstract

摘要： 目的:探讨平阳霉素（pingyangmycin, PYM）、热疗（hyperthermia, HT）及两者联合能否诱导舌鳞癌CAL-27细胞发生免疫原性死亡。方法:对人舌鳞癌细胞CAL-27分别进行PYM、HT及两者联合处理,采用CCK-8实验、Annexin V/PI联合染色、流式细胞术及酶联免疫吸附实验探讨不同处理方式对细胞增殖、凋亡、CRT膜表达及HMGB1分泌的影响。采用SPSS 20.0软件包对数据进行统计学分析。结果:PYM抑制CAL-27细胞活性,且呈浓度依赖性（P<0.05）。PYM及HT均使CRT膜表达率升高;两者联合应用,细胞凋亡、CRT膜表达率及HMGB1分泌均较未处理组、单纯化疗组及单纯HT组升高,差异有统计学意义（P<0.05）。结论:PYM化疗及HT均能诱导舌鳞癌CAL-27细胞CRT由胞内至膜表面转位,并促进HMGB1分泌;两者联合应用,无论在诱导凋亡还是在诱导免疫原性死亡方面,效果优于单纯PYM化疗组。

关键词: 舌, 鳞状细胞癌, 热疗, 热化疗, 免疫原性, 钙网蛋白, 高迁徙族蛋白B1

Abstract: PURPOSE： To investigate whether pingyangmycin(PYM), hyperthermia(HT) and their combination could induce immunogenic cell death of CAL-27 cells. METHODS: CCK-8 assay, flow cytometry and ELISA assay were used to detect the effect of different treatments on CAL-27 cell proliferation, apoptosis, membrane expression rate of CRT and HMGB1 secretion. SPSS 20.0 software package was used for statistical analysis of the data. RESULTS: PYM inhibited CAL-27 cell activity in a concentration-dependent manner (P<0.05). The membrane expression rate of CRT after PYM treatment and HT treatment were significantly higher than that in the untreated group. After treatment of PYM combined with HT, apoptosis, CRT membrane expression rate and HMGB1 secretion were all increased significantly compared with untreated group, chemotherapy group and HT group (P<0.05). CONCLUSIONS: PYM chemotherapy and HT could induce CRT translocation from intracellular to membrane surface of CAL-27 cells, and combined application of PYM and HT was significantly better than that of PYM alone in inducing apoptosis and immunogenic cell death.

Key words: Tongue, Squamous cell carcinoma, Hyperthermia, Thermochemo-therapy, Immunogenicity, Calreticulin, HMGB1

中图分类号:

R739.8

孙巧珍, 石凡, 罗丹, 徐婷, 王升志. 热化疗诱导舌鳞癌CAL-27细胞免疫原性死亡的实验研究[J]. 中国口腔颌面外科杂志, 2020, 18(2): 111-116.

SUN Qiao-zhen, SHI Fan, LUO Dan, XU Ting, WANG Sheng-zhi. Study on immunogenic cell death induced by thermo-chemotherapy in CAL-27 cells of tongue squamous cell carcinoma[J]. China J Oral Maxillofac Surg, 2020, 18(2): 111-116.

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热化疗诱导舌鳞癌CAL-27细胞免疫原性死亡的实验研究

Study on immunogenic cell death induced by thermo-chemotherapy in CAL-27 cells of tongue squamous cell carcinoma

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