间歇性鼻阻塞对幼年大鼠髁突软骨细胞成软骨作用的早期影响

doi:10.19438/j.cjoms.2018.02.008

中国口腔颌面外科杂志 ›› 2018, Vol. 16 ›› Issue (2): 138-143.doi: 10.19438/j.cjoms.2018.02.008

间歇性鼻阻塞对幼年大鼠髁突软骨细胞成软骨作用的早期影响

孙蕙珺, 王晓玲, 朱妍菲, 于倩, 聂萍, 朱敏

上海交通大学医学院附属第九人民医院·口腔医学院口腔颅颌面科,上海市口腔医学重点实验室, 上海市口腔医学研究所,国家口腔疾病临床研究中心,上海 200011;

收稿日期:2017-07-15 修回日期:2017-10-11 出版日期:2018-03-20 发布日期:2018-04-08
通讯作者: 朱敏,E-mail：doctorzhumin@126.com
作者简介:孙蕙珺（1991-）,女,硕士,E-mail：tina_s0327@126.com
基金资助:
上海市科学技术委员会实验动物研究项目（11140902001）

Intermittent nasal obstruction causes early effects on chondrogenesis of mandibular condylar chondrocytes

SUN Hui-jun, WANG Xiao-ling, ZHU Yan-fei, YU Qian, NIE Ping, ZHU Min

Department of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Lab of Stomatology & Shanghai Research Institute of Stomatology; National Clinical Research Center of Stomatology. Shanghai 200011, China;

Received:2017-07-15 Revised:2017-10-11 Online:2018-03-20 Published:2018-04-08

摘要/Abstract

摘要： 目的: 建立幼年大鼠双侧间歇性鼻阻塞动物模型,探讨鼻阻塞对髁突软骨细胞（mandibular condylar chondrocytes,MCCs）成软骨作用的早期影响。 方法: 64只4周龄SD大鼠随机分为4组,每组16只。A组：对照组,不做处理;B组：间歇性鼻阻塞引起开口呼吸4 d后,去除鼻阻塞因素;C组：间歇性鼻阻塞引起开口呼吸8 d后,去除鼻阻塞因素;D组：间歇性鼻阻塞引起开口呼吸16 d。鼻阻塞时间为每天8：00-16：00。在建模第4、8、12、16天,分别取4组大鼠双侧髁突,进行MCCs体外培养并鉴定,RT-PCR法检测4组MCCs成软骨标志基因（SOX9、COL2a、ACAN、PTHrp）表达的差异。采用 SPSS19.0软件包对实验数据进行统计学分析。 结果: 与对照组相比,4 d时B组、C组和D组SOX9、COL2a以及ACAN表达量均显著下降（P<0.05）,但PTHrp表达量显著升高（P<0.05）。8 d时,B组、C组和D组SOX9、COL2a、ACAN、PTHrp表达量均升高,且B组较C组和D组升高更显著（P<0.05）。12 d时,B、C和D组PTHrp的表达仍显著升高（P<0.05）,COL2a、ACAN的表达与对照组持平或降低。16 d时,C组COL2a、ACAN、PTHrp表达量较对照组显著升高（P<0.05）,B组和D组各项基因表达与对照组持平或降低。 结论: 幼年大鼠在早期双侧间歇性鼻阻塞致开口呼吸的情况下,髁突软骨细胞成软骨能力呈现先下降,随后代偿性升高,最终仍表现为下降的趋势。早期去除鼻阻塞因素后,髁突软骨细胞成软骨能力可以短期代偿性增强,但长期作用仍然是成软骨能力降低。

关键词: 鼻阻塞, 开口呼吸, 下颌骨, 髁突软骨细胞, 成软骨

Abstract: PURPOSE: In this study, we investigated the early effects of intermittent nasal obstruction on chondrogenesis of mandibular condylar chondrocytes (MCCs) via animal model. METHODS: Sixty four 4-week-old SD rats were randomly divided into 4 groups, each group contained 16 rats. Group A: control group; Group B: 4-day mouth breathing caused by nasal obstruction; Group C: 8-day mouth breathing caused by nasal obstruction; Group D: 16-day mouth breathing caused by nasal obstruction. The time of nasal obstruction was from 8:00 to 16:00 every day. Bilateral condylar MCCs were harvested and cultured in vitro, on 4th day, 8th day, 12th day and 16th day, respectively. Toluidine blue staining was used to identify MCCs,real-time polymerase chain reaction (RT-PCR) was used to detect the difference of gene expression of MCCs chondrogenic marker genes (SOX9,COL2a,ACAN,PTHrp). The data were statistically analyzed using SPSS19.0 software package. RESULTS: On 4th day of modeling, the expression of SOX9, COL2a and ACAN was lower in group B, C and D compared with group A (P<0.05); but the expression of PTHrp was higher in group B, C and D compared with group A (P<0.05). On 8th day of modeling, the expression of SOX9,COL2a,ACAN and PTHrp was higher in group B, C and D, but much higher in group B than in group C and D(P<0.05). On 12th day of modeling, group B, C and D showed a higher expression of PTHrp (P<0.05), and an equal or lower expression of COL2a, ACAN. On 16th day of modeling, the expression of COL2a, ACAN and PTHrp was increased in group C than in group A(P<0.05). CONCLUSIONS: It is suggested that during mouth breathing caused by nasal obstruction in young rats, the chondogenisis of MCCs decreased followed by a compensatory increase and finally decrease. Furthermore, early removal of nasal obstruction can cause a compensatory increase of MCCs chondrogenisis, but still causes a long-term negative influence.

Key words: Nasal obstruction, Mouth breathing, Mandibular, Mandibular condylar chondrocytes, MCCs, Chondrogenesis

中图分类号:

R782.2

孙蕙珺, 王晓玲, 朱妍菲, 于倩, 聂萍, 朱敏. 间歇性鼻阻塞对幼年大鼠髁突软骨细胞成软骨作用的早期影响[J]. 中国口腔颌面外科杂志, 2018, 16(2): 138-143.

SUN Hui-jun, WANG Xiao-ling, ZHU Yan-fei, YU Qian, NIE Ping, ZHU Min. Intermittent nasal obstruction causes early effects on chondrogenesis of mandibular condylar chondrocytes[J]. China J Oral Maxillofac Surg, 2018, 16(2): 138-143.

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间歇性鼻阻塞对幼年大鼠髁突软骨细胞成软骨作用的早期影响

Intermittent nasal obstruction causes early effects on chondrogenesis of mandibular condylar chondrocytes

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