兔血管化面神经移植动物模型的建立

中国口腔颌面外科杂志 ›› 2014, Vol. 12 ›› Issue (1): 20-24.

兔血管化面神经移植动物模型的建立

朱云¹, 刘胜文¹, 李光辉¹, 田臻², 张陈平¹, 杨雯君¹

1.上海交通大学医学院附属第九人民医院·口腔医学院口腔颌面-头颈肿瘤科,2.口腔病理科,上海市口腔医学重点实验室,上海 200011

收稿日期:2013-04-03 修回日期:2013-04-22 出版日期:2014-02-10 发布日期:2014-02-10
通讯作者: 杨雯君,E-mail：ywjdoctor@hotmail.com
作者简介:朱云(1987-),男,硕士研究生,E-mail:zhuyunoms@gmail.com
基金资助:
上海市教育委员会科研创新项目(14ZZ104); 上海交通大学医学院大学生创新性实验项目(2012054)

A model of vascularized nerve graft for facial nerve repair in rabbit

ZHU Yun¹, LIU Sheng-wen¹, LI Guang-hui¹, TIAN Zhen², ZHANG Chen-ping¹, YANG Wen-jun¹

Department of Oromaxillofacial Head and Neck Oncology, 2.Department of Oral Pathology, Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2013-04-03 Revised:2013-04-22 Online:2014-02-10 Published:2014-02-10
Supported by:
Supported by Innovation Program of Shanghai Municipal Education Commission (14ZZ104) and Innovation Experimental Program for Medical Students of Shanghai Jiao Tong University School of Medicine (2012054).

摘要/Abstract

摘要： 目的: 介绍一种兔血管化面神经移植动物模型建立的方法。方法：选用15只新西兰白兔,带动静脉血管蒂的耳大神经移植组6只,行血管化神经移植修复面神经缺损；游离耳大神经移植组6只,行游离神经移植修复面神经缺损；空白组3只,面神经缺损后不行修复。术后第1、4个月,分别观察兔面部形态的变化,术后4个月行面神经电生理检测,处死后取面神经移植段标本作病理学、免疫组织化学等检测,应用SPSS 13.0软件包对数据进行统计学分析。结果：面神经移植术后,兔手术侧上唇形态及功能均有一定恢复,血管化神经移植组的结果均优于对照组。在神经移植段内均可见轴突再生,良好的血供促进轴突再生。结论：本实验提供了一种操作容易、稳定可靠的兔血管化面神经移植模型,对面神经移植修复的研究有一定价值。

关键词: 血管化神经移植, 面神经, 轴突再生

Abstract: PURPOSE: To develop a new vascularized nerve graft model for facial nerve repair. METHODS: Fifteen New Zealand white rabbits were used in this study. The great auricular nerve of ear was designed as a vascularized graft to serve as a model. VNG group (n=6) received vascularized facial nerve graft, FNG group (n=6) received free nerve graft, control group (n=3) were blank rabbits. The results were evaluated with analysis of facial performance, histological and immunohistochemical studies and electrophysiological monitoring. SPSS 13.0 software package was used for data analysis. RESULTS: At 4-month following facial nerve repair, facial nerve function recovery was observed and analyzed, VNG group was better than FNG group. Through the 20mm facial nerve graft, regenerated nerve fibers were seen, good vascularation facilitated more axons regeneration. CONCLUSIONS: This study demonstrates a new feasibility of vascularized nerve graft in a rabbit model. It has obvious operability and reliability compared with the other models, and the model is useful for future investigation in facial nerve repair.

Key words: Vascularized nerve graft, Facial nerve, Axon regeneration

中图分类号:

R745.1.2

朱云, 刘胜文, 李光辉, 田臻, 张陈平, 杨雯君. 兔血管化面神经移植动物模型的建立[J]. 中国口腔颌面外科杂志, 2014, 12(1): 20-24.

ZHU Yun, LIU Sheng-wen, LI Guang-hui, TIAN Zhen, ZHANG Chen-ping, YANG Wen-jun. A model of vascularized nerve graft for facial nerve repair in rabbit[J]. China J Oral Maxillofac Surg, 2014, 12(1): 20-24.

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