中国口腔颌面外科杂志

中国口腔颌面外科杂志 ›› 2024, Vol. 22 ›› Issue (1): 73-77.doi: 10.19438/j.cjoms.2024.01.013

• 临床总结 • 上一篇    下一篇

槐角提取物对颌骨骨髓间充质干细胞成骨向分化的影响

林惠1#, 高云飞2#, 刘晶3,*, 马旭辉4,*   

  1. 1.上海市儿童医院,上海交通大学医学院附属儿童医院 口腔科,上海 200062;
    2.上海市第八人民医院 口腔科,上海 200030;
    3.上海交通大学医学院附属第九人民医院 口腔第二门诊,上海 200030;
    4.口腔颌面-头颈肿瘤科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心, 上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011
  • 收稿日期:2023-10-15 修回日期:2023-11-29 出版日期:2024-01-20 发布日期:2024-02-05
  • 通讯作者: 马旭辉,E-mail: dr_mxh@126.com;刘晶,E-mail: lj3232475@163.com。*共同通信作者

Effects of Sophora japonica extract on osteogenic differentiation of bone marrow mesenchymal stem cells

LIN Hui1, GAO Yun-fei2, LIU Jing3, MA Xu-hui4   

  1. 1. Department of Dentistry, Shanghai Children's Hospital, Shanghai Jiao Tong University. Shanghai 200062;
    2. Department of Dentistry, Shanghai Eighth People's Hospital. Shanghai 200030;
    3. The Second Dental Center, Shanghai Eighth People's Hospital. Shanghai 200030;
    4. Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology. Shanghai 200011, China
  • Received:2023-10-15 Revised:2023-11-29 Online:2024-01-20 Published:2024-02-05

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摘要： 目的: 探讨槐角提取物对颌骨骨髓间充质干细胞 (jaw bone marrow mesenchymal stem cells,jBMSCs)成骨分化的影响。方法: 采用CCK-8法检测10、25、50、100 μmol/L槐角提取物溶液对jBMSCs增殖的影响,采用BCIP/NBT碱性磷酸酯酶显色、茜素红染色检测10、25、50 μmol/L槐角提取物溶液对jBMSCs成骨分化水平的影响,采用蛋白免疫印迹试验( Western blot) 和实时定量荧光PCR检测槐角提取物溶液对jBMSCs成骨分化相关分子的蛋白和mRNA表达水平的影响。采用GraphPad Prism 9软件包对数据进行统计学分析。结果: 100 μmol/L槐角提取物溶液对jBMSCs 有明显毒性作用（P<0.0001）,10、25和50 μmol/L浓度不影响细胞增殖（P>0.05）。与对照组相比,10、25和50 μmol/L组的碱性磷酸酶活性、钙结节数量、成骨分化相关分子的蛋白和mRNA表达水平均逐渐升高。结论: 10、25、50 μmol/L浓度的槐角提取物能促进jBMSCs成骨向分化。

关键词: 槐角提取物, 颌骨骨髓间充质干细胞, 成骨向分化, 细胞增殖

Abstract: PURPOSE: To investigate the effect of Sophora japonica extract on osteogenic differentiation of jaw bone marrow mesenchymal stem cells (jBMSCs). METHODS: The effects of 10, 25, 50 and 100 μmol/L Sophora japonica extract solutions on proliferation of jBMSCs were detected by CCK-8. BCIP/NBT alkaline phosphatase and alizarin red staining were used to detect the effect of 10, 25, 50 μmol/L extracts of Sophora on osteogenic differentiation of jBMSCs. Western blot analysis and real-time quantitative PCR were used to detect the effects of Sophora japonica extract solution on protein and mRNA expression levels of osteogenic differentiation-related genes of jBMSCs. GraphPad Prism 9 software package was used for data analysis. RESULTS: The experimental results showed that 100 μmol/L Sophora japonica extract solution had obvious toxic effect on jBMSCs(P<0.0001); while 10, 25 and 50 μmol/L Sophora japonica extract solution did not affect cell proliferation(P>0.05). Compared with the control group, alkaline phosphatase activity, number of calcium nodules, and protein and mRNA expression levels of osteogenic differentiation-related molecules in the 10, 25 and 50 μmol/L groups were gradually increased. CONCLUSIONS: The extract of Sophora japonica on 10, 25, 50 μmol/L can enhance the osteogenic differentiation of jBMSCs.

Key words: Sophora japonica extract, Jaw bone marrow mesenchymal stem cells, Osteogenic differentiation, Cell proliferation

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