关键词: 西地那非, 淋巴管畸形, 内皮细胞

Abstract: PURPOSE: To explore the effect and mechanism of sildenafil on proliferation of human lymphatic malformations endothelial cells (HLMECs) in vitro. METHODS: Tissue block culture method was used to culture HLMECs in vitro, which were identified by immunocytochemistry (CD31 and LYVE-1). Subsequent studies were performed using third-generation cells, the cells were treated with sildenafil at the concentration of 1, 2, 5 and 10 μmol/L, respectively, cell morphology was observed under an inverted microscope; cell proliferation activity was detected by MTT and EDU staining, and the expression level of vascular endothelium growth factor C (VEGFC) and its receptor VEGFR-3 was detected by Western blot. Independent-samples t test and ANOVA (LSD method) were performed using SPSS 21.0 software package for statistical analysis. RESULTS: Cells cultured by tissue block method revealed both positive CD31 and LYVE-1 staining. After treated with sildenafil, the cell morphology was changed and the density of the cells was decreased. MTT and EDU staining reflected that the cell proliferation was significantly inhibited by sildenafil at the concentration of 2 μmol/L (P<0.05). There was also decreased level of VEGFC and VEGFR-3, detected by Western blot. CONCLUSIONS: Sildenafil could inhibit the proliferation of HLMECs in vitro. The mechanism might be relate to the regulation of VEGFC /VEGFR-3.

Key words: Sildenafil, Lymphatic malformation, Endothelial cells