miR-181a抑制唾液腺腺样囊性癌侵袭、转移机制的实验研究

中国口腔颌面外科杂志 ›› 2015, Vol. 13 ›› Issue (1): 11-15.

miR-181a抑制唾液腺腺样囊性癌侵袭、转移机制的实验研究

何倩婷, 刘中华, 招洛丹, 赵婷婷, 王安训

中山大学附属第一医院口腔科,广东广州 510080

收稿日期:2014-06-30 出版日期:2015-02-10 发布日期:2015-03-12
通讯作者: 王安训,E-mail：Anxunwang@yahoo.com E-mail:qiantinghe@hotmail.com
作者简介:何倩婷（1984-）,女,硕士

The mechanism of miR-181a inhibiting invasion and migration of salivary adenoid cystic carcinoma: an in vitro study

HE Qian-ting, LIU Zhong-hua, ZHAO Luo-dan, ZHAO Ting-ting, WANG An-xun

Department of Dentistry, First Hospital of Sun Yat-sen University. Guangzhou 510080, Guangdong Province, China

Received:2014-06-30 Online:2015-02-10 Published:2015-03-12
About author:Supported by Science and Technology Planning Project of Guangdong Province (2012B031800144), and the New Technology, New Project of Nanfang Hospital (201118)
Supported by:
广东省科技计划项目（2012B031800144）; 南方医院新技术、新项目（201118）

摘要/Abstract

摘要： 目的探讨miR-181a在唾液腺腺样囊性癌细胞侵袭、转移中的作用。方法利用QRT-PCR检测miR-181a在一对不同侵袭、迁移能力细胞株中的表达水平。过表达或沉默miR-181a在SACC-LM和SACC-83细胞中的表达。利用激光共聚焦显微镜观察转染后细胞骨架的改变。Western免疫印迹检测转染后侵袭、转移相关因子的表达。通过尾静脉注射miR-181a mimics和mimics NC细胞,建立裸鼠肺转移模型。采用SPSS17.0软件包对数据进行统计学处理。结果 QRT-PCR结果显示,miR-181a在低侵袭、迁移能力SACC-83细胞中的表达量显著高于高侵袭、迁移能力的SACC-LM细胞。SACC-LM细胞转染miR-181a mimics后,细胞呈梭形,Slug、pERK1/2、ERK1/2表达水平下调;SACC-83细胞转染miR-181a LNA后,细胞变圆,体积变小,Slug、pERK1/2、ERK1/2表达水平升高。裸鼠肺转移模型miR-181a mimics组,细胞形成肺转移灶面积显著小于mimics NC组（P<0.05）。结论 miR-181a能抑制SACC的侵袭和转移。

关键词: miR-181a, 唾液腺腺样囊性癌, 侵袭, 转移

Abstract: PURPOSE: To investigate the effect of miR-181a on invasion and migration in salivary adenoid cystic carcinoma (SACC). METHODS: QRT-PCR analysis was used to detect the expression of miR-181a in the paired SACC cell lines with different invasive and migration ability. The SACC cell lines SACC-LM and SACC-83 were transfected with miR-181a mimics and miR-181a LNA, respectively. Confocal microscopy was used to observe the changes of cytoskeleton after transfection. Western blot analysis was performed to demonstrate the invasion and metastasis related genes after transfection. Lung metastasis model in nude mice was established to analyze the effect of miR-181a on lung metastatic ability of SACC cell lines after transfection. The data was analyzed using SPSS 17.0 software package. RESULTS: QRT-PCR analysis showed that low invasive and migration ability cell line (SACC-83) expressed higher miR-181a than higher invasive and migration ability cell line (SACC-LM). Ectopic transfection of the miR-181a mimics to the SACC-LM cells led to decreased gene expression of Slug, pERK1/2, ERK1/2 and cells turned to be spindle. When the SACC-83 cells were treated with miR-181a LNA, the gene expression of Slug, pERK1/2, ERK1/2 increased and cells turned to be round. Lung metastasis model in nude mice showed that compared with the mimics NC group, lung metastatic lesion size of miR-181a mimics group was significantly smaller (P<0.05). CONCLUSIONS: miR-181a can suppress the invasion and metastasis of SACC.

Key words: miR-181a, Salivary adenoid cystic carcinoma, Invasion, Metastasis

中图分类号:

R739.87

何倩婷, 刘中华, 招洛丹, 赵婷婷, 王安训. miR-181a抑制唾液腺腺样囊性癌侵袭、转移机制的实验研究[J]. 中国口腔颌面外科杂志, 2015, 13(1): 11-15.

HE Qian-ting, LIU Zhong-hua, ZHAO Luo-dan, ZHAO Ting-ting, WANG An-xun. The mechanism of miR-181a inhibiting invasion and migration of salivary adenoid cystic carcinoma: an in vitro study[J]. China J Oral Maxillofac Surg, 2015, 13(1): 11-15.

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