基于Cre/Loxp重组酶系统的谱系示踪技术在成骨改建研究中的应用进展

doi:10.19438/j.cjoms.2021.06.015

中国口腔颌面外科杂志 ›› 2021, Vol. 19 ›› Issue (6): 562-567.doi: 10.19438/j.cjoms.2021.06.015

基于Cre/Loxp重组酶系统的谱系示踪技术在成骨改建研究中的应用进展

徐宁蔚^1,*, 王晶^1,*, 龚佳静¹, 张瑾¹, 金安婷², 江凌勇²

1. 上海交通大学医学院附属第九人民医院·口腔医学院,
2. 口腔颅颌面科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海 200011

出版日期:2021-12-25 发布日期:2022-03-02
通讯作者: 江凌勇,E-mail：247416218@qq.com
作者简介:徐宁蔚（2000-），女，在读本科生，E-mail：1965859192@qq.com；王晶（2000-），女，在读本科生，E-mail：1537930645@qq.com。*并列第一作者
基金资助:
国家自然科学基金（81570950,81870740; 81800949）; 上海市优秀学术/技术带头人计划（20XD1422300）; 上海市“医苑新星”青年医学人才培养资助计划—杰出青年医学人才项目（HWJRS 2019-72）; 上海交通大学医学院高水平创新团队激励计划-协同创新团队激励项目; 上海交通大学医学院第十四期大学生创新训练计划（1420Y507）; 上海交通大学医学院附属第九人民医院“交叉”研究基金项目·重点项目（JYJC201902）

Application of lineage tracing technique based on Cre/Loxp recombinase system in the study of osteogenic remodeling

XU Ning-wei¹, WANG Jing¹, GONG Jia-jing¹, ZHANG Jin¹, JIN An-ting², JIANG Ling-yong²

1. College of Stomatology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China;
2. Department of Oral and Maxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Online:2021-12-25 Published:2022-03-02

摘要/Abstract

摘要： 细胞谱系示踪技术是研究干细胞特性的重要工具，基于Cre/Loxp重组酶系统的谱系示踪技术是示踪方法的一大突破，组成性重组酶系统实现了报告基因的组织特异性，诱导性重组酶系统在此基础上实现了报告基因表达时间的可控性。研究成骨改建机制涉及成骨细胞系命运，对骨改建研究、组织工程学应用是有重要意义。本文综述了基于Cre/Loxp重组酶系统的谱系示踪技术在研究成骨系细胞分化、来源、转归及其在发育和修复中的功能等方面的应用，并探讨其适用范围、优势和不足。

关键词: 谱系示踪, 成骨改建, Cre/loxp重组酶系统

Abstract: [Summary] Cell lineage tracing technology is an important tool to study the characteristics of stem cells, among which, the one based on Cre/Loxp recombinase system is a great breakthrough. General recombinase system realizes the tissue specificity of reporter gene, based on which, inducible recombinase system makes controlling the expression time of reporter gene possible. The study of the mechanism of osteogenic remodeling is related to the fate of osteoblast lines, which is of great significance to the study of bone remodeling and the application of tissue engineering. This paper reviewed the application of lineage tracing technique based on Cre/Loxp recombinase system in the study of the origin, differentiation, lineage and hierarchy of osteoblast lines and their function in development and repair, and discussed its scope of application, advantages and disadvantages.

Key words: Lineage tracing, Osteogenic remodeling, Cre/Loxp recombinase system

中图分类号:

Q2-33

徐宁蔚, 王晶, 龚佳静, 张瑾, 金安婷, 江凌勇. 基于Cre/Loxp重组酶系统的谱系示踪技术在成骨改建研究中的应用进展[J]. 中国口腔颌面外科杂志, 2021, 19(6): 562-567.

XU Ning-wei, WANG Jing, GONG Jia-jing, ZHANG Jin, JIN An-ting, JIANG Ling-yong. Application of lineage tracing technique based on Cre/Loxp recombinase system in the study of osteogenic remodeling[J]. China J Oral Maxillofac Surg, 2021, 19(6): 562-567.

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基于Cre/Loxp重组酶系统的谱系示踪技术在成骨改建研究中的应用进展

Application of lineage tracing technique based on Cre/Loxp recombinase system in the study of osteogenic remodeling

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