长链非编码RNA CASC15在成釉细胞瘤中的差异表达及生物信息学分析

doi:10.19438/j.cjoms.2021.05.005

中国口腔颌面外科杂志 ›› 2021, Vol. 19 ›› Issue (5): 411-416.doi: 10.19438/j.cjoms.2021.05.005

长链非编码RNA CASC15在成釉细胞瘤中的差异表达及生物信息学分析

何优雅, 张誉, 戴振霖, 林承重, 曹巍, 季彤

上海交通大学医学院附属第九人民医院口腔颌面-头颈肿瘤科,上海交通大学口腔医学院, 国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海 200011

收稿日期:2021-01-13 修回日期:2021-03-11 出版日期:2021-09-20 发布日期:2021-10-20
通讯作者: 季彤,E-mail：jitong70@hotmail.com
作者简介:何优雅（1995-）,女,硕士,住院医师,E-mail：hyy520@163.com
基金资助:
国家自然科学基金（81671009）

Differential expression and bioinformation of lncRNA CASC15 in ameloblastoma

HE You-ya, ZHANG Yu, DAI Zhen-lin, LIN Cheng-zhong, CAO Wei, JI Tong

Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine;; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2021-01-13 Revised:2021-03-11 Online:2021-09-20 Published:2021-10-20

摘要/Abstract

摘要： 目的探讨CASC15在成釉细胞瘤（ameloblastoma, AM）组织中的表达及其在AM发生、发展过程中的作用机制。方法分析8对AM及其正常瘤旁组织二代高通量全转录测序数据筛选出的目的 lncRNA CASC15,采用DEseq2算法筛选与CASC15具有显著差异表达的mRNA;通过与GO数据库以及KEGG数据库中的基因注释进行比对,以及基因本体论（Gene Ontology,GO）分析和信号通路分析。通过计算Pearson和K-core,分析差异表达mRNA相对于CASC15的核心度,构建CASC15与差异mRNA的共表达网络。采用qRT-PCR检测CASC15在AM组织中的表达,采用GraphPad Prism 8软件包对数据进行统计学分析。结果在CASC15相关mRNA中筛选出172个具有差异表达的mRNA。GO分析显示,与CASC15差异共表达的mRNA主要参与细胞增殖、信号转导、迁移和血管生成等生物学行为。信号通路分析显示,与CASC15共表达的mRNA主要参与癌症相关信号通路、Hedgehog、p53等信号通路,破骨细胞分化、细胞周期等途径。共表达网络分析筛选出5个与CASC15有密切联系的高核心度mRNA—FGF18、EPCAM、PTCH1、SHH和GLI1,可用于后续AM复发、侵袭机制的研究。qRT-PCR结果显示,CASC15在AM中高表达,相对于瘤旁正常组织的差异表达倍数为11.41倍（P<0.0001）,相对于牙囊组织中的差异表达倍数为11.72（P<0.0001）。结论 CASC15在AM中高表达,可能通过调控细胞增殖、迁移、细胞周期等参与AM的发生、发展。

关键词: 长链非编码RNA, CASC15, 成釉细胞瘤, 生物信息学, 侵袭性

Abstract: PURPOSE: To explore the expression of CASC15 and its mechanism in the development of ameloblastoma. METHODS: Second-generation high-throughput full-transcription sequencing data of 8 pairs of ameloblastoma and adjacent normal tissues were analyzed to screen out the target lncRNA CASC15. The co-expression mRNA with significant difference from CASC15 was screened by using DEseq2 algorithm. Gene Ontology (GO) analysis and signal pathway analysis were performed by comparing with the gene annotations in the GO database and KEGG database. The expression of CASC15 in ameloblastoma was detected by qRT-PCR. The data were analyzed with GraphPad Prism 8 software package. RESULTS: A total of 172 differentially expressed CASC15-related mRNA were screened out. GO analysis showed that the differentially co-expressed CASC15 mRNA was mainly involved in cell proliferation regulation, cell signal transduction, cell migration, angiogenesis and other biological behaviors. Pathway analysis showed that the co-expressed mRNA of CASC15 was mainly involved in pathway in cancer, Hedgehog, p53 as well as cancer-related signal pathway in osteocyte differentiation, cell cycle and so on. Five high core genes such as FGF18, EPCAM, PTCH1, SHH and GLI1, which were closely related to CASC15, screened by co-expression network analysis, which can be used to explore the mechanism of recurrence and invasion of ameloblastoma. qRT-PCR results showed that CASC15 was highly expressed in ameloblastoma, which was 11.41 times higher than that in adjacent normal tissues (P<0.0001), and 11.72 times higher than that in dental follicle tissues (P<0.0001). CONCLUSIONS: CASC15 is highly expressed in ameloblastoma, which may participate in the occurrence and development of ameloblastoma by regulating cell proliferation, cell migration and cell cycle.

Key words: Long non-coding RNA, CASC15, Ameloblastoma, Bioinformatics, Invasion

中图分类号:

R739.8

何优雅, 张誉, 戴振霖, 林承重, 曹巍, 季彤. 长链非编码RNA CASC15在成釉细胞瘤中的差异表达及生物信息学分析[J]. 中国口腔颌面外科杂志, 2021, 19(5): 411-416.

HE You-ya, ZHANG Yu, DAI Zhen-lin, LIN Cheng-zhong, CAO Wei, JI Tong. Differential expression and bioinformation of lncRNA CASC15 in ameloblastoma[J]. China J Oral Maxillofac Surg, 2021, 19(5): 411-416.

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长链非编码RNA CASC15在成釉细胞瘤中的差异表达及生物信息学分析

Differential expression and bioinformation of lncRNA CASC15 in ameloblastoma

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