缺氧诱导因子1α在成釉细胞瘤中的表达及意义

doi:10.19438/j.cjoms.2018.04.010

中国口腔颌面外科杂志 ›› 2018, Vol. 16 ›› Issue (4): 343-347.doi: 10.19438/j.cjoms.2018.04.010

缺氧诱导因子1α在成釉细胞瘤中的表达及意义

唐渔, 曹巍, 彭灿邦, 刘喆麒, 林承重, 季彤

上海交通大学医学院附属第九人民医院·口腔医学院口腔颌面-头颈肿瘤科,国家口腔疾病临床研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011

收稿日期:2018-03-01 修回日期:2018-04-07 出版日期:2018-07-20 发布日期:2018-08-09
通讯作者: 季彤,E-mail: jitong70@hotmail.com
作者简介:唐渔（1991-）,女,硕士,E-mail:xiaoxiaoyuer925@163.com
基金资助:
国家自然科学基金（81671009）

Relationship between expression of hypoxia-inducible factor 1α and clinical characteristic of recurrent ameloblastoma

TANG Yu, CAO Wei, PENG Can-bang, LIU Zhe-qi, LIN Cheng-zhong, JI Tong

Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology. Shanghai 200011, China

Received:2018-03-01 Revised:2018-04-07 Online:2018-07-20 Published:2018-08-09

摘要/Abstract

摘要： 目的:检测缺氧诱导因子1α（hypoxia-inducible factor 1α,HIF-1α）在成釉细胞瘤中的表达,并探讨其与成釉细胞瘤侵袭和复发的相关性。方法:对5对成釉细胞瘤及对应正常瘤旁组织进行转录组测序,并对60例原发及60例复发患者第1次手术标本行免疫组织化学染色,检测2组患者中HIF-1α蛋白的表达。采用SAS9.3软件包对数据进行统计学分析。结果:5对新鲜标本转录组测序发现,3对标本中HIF-1α及其相关分子转录水平呈明显一致性改变。免疫组织化学染色发现,复发组成釉细胞瘤中,40%的肿瘤HIF-1α染色高于未复发组;复发组中,48.33%的肿瘤slug染色高于未复发组,差异具有统计学意义（P<0.05）。结论:成釉细胞瘤复发与HIF-1α相关,HIF-1α表达高的患者较表达低的患者更容易复发。HIF-1α可能通过调控slug的表达,影响成釉细胞瘤的局部复发。

关键词: HIF-1α, 成釉细胞瘤, Slug

Abstract: PURPOSE:To detect the expression of hypoxia-inducible factor 1α in ameloblastoma and to determine if it was involved in the recurrence of ameloblastoma. METHODS: Five pairs of ameloblastoma and normal para-tissue were used for transcriptional group sequence; 120 formalin-fixed, paraffin-embeded tissue blocks of ameloblastoma specimens were compared at protein level using immunohistochemistry for HIF-1α staining. The data were analyzed with SAS 9.3 software package. RESULTS: The transcriptional level of HIF-1α and its related molecules in 3/5 specimens were changing consistently and obviously after sequencing; the HIF-1α and slug expression levels were significantly higher in recurrent ameloblastoma. About 40% of recurrent ameloblastoma had higher HIF-1α staining than primary ameloblastoma and 48.33% of recurrent ameloblastoma had higher slug staining than primary ameloblastoma (P<0.05). CONCLUSIONS: Higher expression of HIF-1α plays an important role in the recurrence of ameloblastoma.

Key words: HIF-1α, Ameloblastoma, Slug

中图分类号:

R739.8

唐渔, 曹巍, 彭灿邦, 刘喆麒, 林承重, 季彤. 缺氧诱导因子1α在成釉细胞瘤中的表达及意义[J]. 中国口腔颌面外科杂志, 2018, 16(4): 343-347.

TANG Yu, CAO Wei, PENG Can-bang, LIU Zhe-qi, LIN Cheng-zhong, JI Tong. Relationship between expression of hypoxia-inducible factor 1α and clinical characteristic of recurrent ameloblastoma[J]. China J Oral Maxillofac Surg, 2018, 16(4): 343-347.

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缺氧诱导因子1α在成釉细胞瘤中的表达及意义

Relationship between expression of hypoxia-inducible factor 1α and clinical characteristic of recurrent ameloblastoma

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